FoxO1/FKHR Antibody (SU33-01)

Name: FoxO1/FKHR Antibody (SU33-01)
Brand: Novus Biologicals
SKU: NBP2-67463
Price: 449 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-67463

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Zebrafish

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Frozen

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SU33-01 expressed in HEK293

View all FoxO1/FKHR Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide within Human FoxO1/FKHR aa 301-350 / 655. (SwissProt: Q12778 Human; SwissProt: Q9R1E0 Mouse; SwissProt: G3V7R4 Rat)

Localization

Cytoplasm, Nucleus.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for FoxO1/FKHR Antibody (SU33-01)

Western Blot: FoxO1/FKHR Antibody (SU33-01) [NBP2-67463]

Western Blot: FoxO1/FKHR Antibody (SU33-01) [NBP2-67463] - Analysis of FOXO1A on different lysates with Rabbit anti-FOXO1A antibody at 1/500 dilution. Lane 1: Hela cell lysate Lane 2: NIH/3T3 cell lysate ysates/proteins at 10 ug/Lane. Predicted band size: 70 kDa observed band size: 85 kDa Exposure time: 1 minute; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry/ Immunofluorescence: FoxO1/FKHR Antibody (SU33-01) [NBP2-67463]

Immunocytochemistry/Immunofluorescence: FoxO1/FKHR Antibody (SU33-01) [NBP2-67463] - Staining FOXO1A in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: FoxO1/FKHR Antibody (SU33-01) [NBP2-67463]

Immunohistochemistry-Paraffin: FoxO1/FKHR Antibody (SU33-01) [NBP2-67463] - Analysis of paraffin-embedded mouse heart tissue using anti-FOXO1A antibody. Counter stained with hematoxylin.

Immunocytochemistry/ Immunofluorescence: FoxO1/FKHR Antibody (SU33-01) [NBP2-67463]

Immunocytochemistry/Immunofluorescence: FoxO1/FKHR Antibody (SU33-01) [NBP2-67463] - Staining FOXO1A in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/ Immunofluorescence: FoxO1/FKHR Antibody (SU33-01) [NBP2-67463]

Immunocytochemistry/Immunofluorescence: FoxO1/FKHR Antibody (SU33-01) [NBP2-67463] - Staining FOXO1A in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: FoxO1/FKHR Antibody (SU33-01) [NBP2-67463]

Immunohistochemistry-Paraffin: FoxO1/FKHR Antibody (SU33-01) [NBP2-67463] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-FOXO1A antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: FoxO1/FKHR Antibody (SU33-01) [NBP2-67463]

Immunohistochemistry-Paraffin: FoxO1/FKHR Antibody (SU33-01) [NBP2-67463] - Analysis of paraffin-embedded human tonsil tissue using anti-FOXO1A antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: FoxO1/FKHR Antibody (SU33-01) [NBP2-67463]

Immunohistochemistry-Paraffin: FoxO1/FKHR Antibody (SU33-01) [NBP2-67463] - Analysis of paraffin-embedded mouse brain tissue using anti-FOXO1A antibody. Counter stained with hematoxylin.

Western Blot: FoxO1/FKHR Antibody (SU33-01) [NBP2-67463]

Western blot analysis on different lysates with NBP2-67463 at 1/5,000 dilution. Lane 1: THP-1 cell lysate (15 µg/Lane) Lane 2: Jurkat cell lysate (15 µg/Lane) Lane 3: NIH/3T3 cell lysate (15 µg/Lane) Lane 4: PC-12 cell lysate (15 µg/Lane) Lane 5: Mouse brain tissue lysate (20 µg/Lane) Lane 6: Rat brain tissue lysate (20 µg/Lane) Predicted band size: 70 kDa Observed band size: 100 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP2-67463 at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

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Applications for FoxO1/FKHR Antibody (SU33-01)

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:1000-1:2000

Application Notes

IHC-Fr usage was reported in scientific literature (PMID: 31196960).

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FoxO1/FKHR

FoxO1, also called FKHR (forkhead in rhabdomyosarcoma), is a ubiquitously expressed member of the forkhead box O family of winged helix transcription factors. In neurons, it is activated in response to stress, translocating to the nucleus where it promotes apoptosis and blocks proliferation. In insulin-responsive tissues, nutrient abundance triggers phosphorylation by AKT that blocks nuclear translocation and activity.

Long Name

Forkhead Box O1

Alternate Names

FKH1, FKHR

Gene Symbol

FOXO1

Additional FoxO1/FKHR Products

Product Documents for FoxO1/FKHR Antibody (SU33-01)

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Certificate of Analysis

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Product Specific Notices for FoxO1/FKHR Antibody (SU33-01)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.