Glutathione Synthetase Antibody (OTI1B8)

Novus Biologicals | Catalog # NBP2-03351

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry

Cited:

Immunohistochemistry, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1B8
View all Glutathione Synthetase Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human GSS (NP_000169) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

52.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Glutathione Synthetase Antibody (OTI1B8)

Western Blot: Glutathione Synthetase Antibody (OTI1B8) [NBP2-03351]

Western Blot: Glutathione Synthetase Antibody (OTI1B8) [NBP2-03351]

Western Blot: Glutathione Synthetase Antibody (1B8) [NBP2-03351] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Glutathione Synthetase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Glutathione Synthetase.
Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody (1B8) [NBP2-03351] Staining of paraffin-embedded Human prostate tissue using anti-Glutathione Synthetase mouse monoclonal antibody.
Flow Cytometry: Glutathione Synthetase Antibody (OTI1B8) [NBP2-03351]

Flow Cytometry: Glutathione Synthetase Antibody (OTI1B8) [NBP2-03351]

Flow Cytometry: Glutathione Synthetase Antibody (1B8) [NBP2-03351] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Glutathione Synthetase antibody, and then analyzed by flow cytometry.
Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody (OTI1B8) [NBP2-03351]

Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody (OTI1B8) [NBP2-03351]

Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody (1B8) [NBP2-03351] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-Glutathione Synthetase mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody (OTI1B8) [NBP2-03351]

Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody (OTI1B8) [NBP2-03351]

Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody (1B8) [NBP2-03351] - Staining of paraffin-embedded Human Kidney tissue using anti-Glutathione Synthetase mouse monoclonal antibody.

Applications for Glutathione Synthetase Antibody (OTI1B8)

Application
Recommended Usage

Flow Cytometry

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:2000

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.51 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Glutathione Synthetase

Glutathione is important for a variety of biological functions, including protection of cells from oxidative damage by free radicals, detoxification of xenobiotics, and membrane transport. The protein encoded by this gene functions as a homodimer to catalyze the second step of glutathione biosynthesis, which is the ATP-dependent conversion of gamma-L-glutamyl-L-cysteine to glutathione. Defects in this gene are a cause of glutathione synthetase deficiency. [provided by RefSeq].

Alternate Names

EC 6.3.2.3, Glutathione synthase, glutathione synthetase, GSH synthetase, GSHS, GSH-S, MGC14098

Entrez Gene IDs

2937 (Human)

Gene Symbol

GSS

Additional Glutathione Synthetase Products

Product Documents for Glutathione Synthetase Antibody (OTI1B8)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Glutathione Synthetase Antibody (OTI1B8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Glutathione Synthetase Antibody (OTI1B8)

Customer Reviews for Glutathione Synthetase Antibody (OTI1B8)

There are currently no reviews for this product. Be the first to review Glutathione Synthetase Antibody (OTI1B8) and earn rewards!

Have you used Glutathione Synthetase Antibody (OTI1B8)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies