HO-1/HMOX1/HSP32 Antibody (HO-1-1) - BSA Free

Western Blot: HO-1/HMOX1/HSP32 Antibody (HO-1-1) - BSA Free [NBP1-97507] -

Effect of Sepsis with Isoflurane or Propofol anesthesia on Liver HIF1a, Bcl2, iNOS and HO-1, Protein expression levels. (A) HIF-1 alpha protein was increased in both anesthetic groups by sepsis (Isoflurane +Sepsis, **p = 0.004 and Propofol + Sepsis, *p = 0.04). (B) Bcl-2 protein was increased in Isoflurane +Sepsis group (**p = 0.004) and no significant change in Propofol + Sepsis (*p = 0.104). (C) Inducible nitric oxide synthase [iNOS, (**p = 0.002)] and (D) Heme oxygenase-1 (HO-1, ***p = 0.0006) was increased in Isoflurane +Sepsis group. Whereas, in animals anesthetized with Propofol, three organ-protective proteins, Bcl-2, iNOS, and HO-1 expression was unchanged significantly. Data are shown in mean +/- S.D, n = 12. *denotes a statistically significant (P 0.05) difference between animals exposed to sepsis and controls. Statistical differences between results were evaluated using the t-test. “ns" (in graphs) represents not statistically significant. “ns" (in immunoblots) represents non-specific signal. “◂" represents specific signal. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33392215), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: HO-1/HMOX1/HSP32 Antibody (HO-1-1) - BSA Free [NBP1-97507] -

Protein expression data (upper) and representative raw data (lower) for animals anesthetized with isoflurane (bcl-2 [a median + IQR], HIF-1 alpha [b mean + 95% confidence interval (CI)], HO-1 [c mean + 95% CI for isoflurane, median + IQR for propofol], iNOS [d mean + 95% CI for isoflurane, median + IQR for propofol) *denotes a statistically significant difference between animals exposed to sepsis and controls Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31307382), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: HO-1/HMOX1/HSP32 Antibody (HO-1-1) - BSA Free [NBP1-97507] -

KEAP1 modulates ferroptosis through NRF2/HO‐1 pathway in hiPSC‐derived cINs. (A) Western blot showed the expression of NRF2 and HO‐1. (B) Quantification of Fe2⁺ levels, using an iron content determination assay. (C) Measurement of MDA levels using an MDA assay kit. (D) Quantification of GSH levels using a GSH assay kit. (E) Assessment of GPX4 activity using an ELISA kit. (F) GPX4 mRNA and protein were evaluated by qRT‐PCR and Western blot analysis, respectively. (G) Measurement of lipid oxidation using C11‐BODIPY staining, a dye specific for detecting lipid reactive oxygen species. (H) Quantification of inflammatory cytokines in the cell culture supernatant using ELISA kits. *p < 0.05 and **p < 0.01 show statistical significance when compared to SCZ‐pcDNA KEAP1 group. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/40021790), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: HO-1/HMOX1/HSP32 Antibody (HO-1-1) - BSA Free [NBP1-97507] -

ARE-primed PD-MSCs increased antioxidant and anti-inflammatory effects in ARPE19 cells. (A) Oxidative stress levels evaluated in ARPE19 cells using MitoSOX/MitoTracker staining and (B) intensity quantified by image J. The gray bar, gray hatch bar, black bar, black hatch bar means ARPE19 cells with H2O2 and ARE low, ARPE19 cells with with H2O2, ARE low and PD-MSCs, ARPE19 cells with H2O2 and ARE high and ARPE19 cells with with H2O2, ARE high and PD-MSCs. The mRNA levels of (C) SOD1 and (D) HMOX1 analyzed by qRT-PCR. The protein levels of (E) SOD1 and (F) HMOX1 analyzed by Western blot. The mRNA levels of (G) TGFB1, (H) IL6 analyzed by qRT-PCR. The protein levels of (I) TGFB1 and (J) IL6 analyzed by Western blot. Scale bar = 200 μm. Each experiment was replicated three times, resulting in a total sample size of three (n = 3). The data represent the mean +/- SEM. Statistical significance was determined by using one-way ANOVA and Tukey’s post hoc test for the comparison of groups, * p < 0.05. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/39056810), licensed under a CC-BY license. Not internally tested by Novus Biologicals.