Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 504425
View all ADAM23 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human ADAM23
Ser60-His585
Accession # O75077

Specificity

Detects human ADAM23 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human ADAM23 Antibody

ADAM23 antibody in Human Spleen by Immunohistochemistry (IHC-P).

ADAM23 in Human Spleen.

ADAM23 was detected in immersion fixed paraffin-embedded sections of human spleen using 15 µg/mL Mouse Anti-Human ADAM23 Monoclonal Antibody (Catalog # MAB4974) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human ADAM23 Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human spleen subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)

Reviewed Applications

Read 3 reviews rated 3.7 using MAB4974 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ADAM23

ADAM23 (a disintegrin and metalloprotease domain 23; also MDC3) is a 100 kDa member of the M12B peptidase family of enzymes. It is expressed on fetal neurons in the hippocampus and cerebellum, and serves as a counter-receptor for alpha v beta 3 integrin. The human ADAM23 proprecursor is a 773 amino acid (aa) type I transmembrane (TM) protein. It contains a 227 aa cleavable proregion (aa 60‑286) and a 506 aa extracellular domain (ECD) (aa 287‑792) that is part of a 70 kDa mature molecule. The ECD contains a nonfunctional metalloprotease domain (aa 299‑494), an integrin-binding disintegrin region (aa 511‑585), and a Cys‑rich domain (aa 589‑611). Two splice variants exist. One shows an in-frame 46 aa substitution for aa 787‑832 that generates a soluble form, while a second shows an in‑frame 31 aa TM substitution for aa 787‑817. Over aa 60‑585, human ADAM23 shares 92% aa sequence identity with mouse ADAM23.

Long Name

A Disintegrin and Metalloproteinase Domain 23

Alternate Names

MDC-L, MDC3

Entrez Gene IDs

8745 (Human); 23792 (Mouse); 301460 (Rat)

Gene Symbol

ADAM23

UniProt

O75077

Additional ADAM23 Products

Product Documents for Human ADAM23 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human ADAM23 Antibody

For research use only

Related Research Areas

Metalloproteases and Regulators

Customer Reviews for Human ADAM23 Antibody (3)

3.7 out of 5
3 Customer Ratings
5 Stars
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4 Stars
67%
3 Stars
33%
2 Stars
0%
1 Stars
0%

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Customer Images

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  • Human ADAM23 Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 03/11/2019
    Human ADAM23 Antibody MAB4974
  • Human ADAM23 Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human and Mouse
    Verified Customer | Posted 11/08/2018
  • Human ADAM23 Antibody
    Name: Anonymous
    Application: Microarray
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 11/02/2018

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Protocols

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