Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 248621
View all CCR9 Primary Antibodies »

Product Specifications

Immunogen

Human CCR9 transfectants
Met1-Leu369
Accession # P51686

Specificity

Detects human CCR9.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human CCR9 Antibody

Detection of CCR9 antibody in Human MOLT-4 cells antibody by Flow Cytometry.

Detection of CCR9 in Human MOLT-4 cells by Flow Cytometry.

MOLT-4 human acute lymphoblastic leukemia cell line was stained with Mouse Anti-Human CCR9 Monoclonal Antibody (Catalog # MAB1791, filled histogram) or Mouse IgG2A Flow Cytometry Isotype Control (Catalog # MAB003) followed by PE-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

CCR9 antibody in MOLT-4 Human Cell Line by Immunocytochemistry (ICC).

CCR9 in MOLT‑4 Human Cell Line.

CCR9 was detected in immersion fixed MOLT-4 human acute lymphoblastic leukemia cell line using Mouse Anti-Human CCR9 Monoclonal Antibody (Catalog # MAB1791) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Detection of CCR9 antibody in HEK293 Human Cell Line Transfected with Human CCR9 and eGFP antibody by Flow Cytometry.

Detection of CCR9 in HEK293 Human Cell Line Transfected with Human CCR9 and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with human CCR9 and eGFP was stained with (A) Mouse Anti-Human CCR9 Monoclonal Antibody (Catalog # MAB1791) or (B) Mouse IgG2A isotype control antibody (Catalog # MAB003) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Applications for Human CCR9 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: MOLT-4 human acute lymphoblastic leukemia cell line and HEK293 Human Cell Line Transfected with Human CCR9 and eGFP

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed MOLT-4 human acute lymphoblastic leukemia cell line

Flow Cytometry Panel Builder

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CCR9

CCR9 is a G protein-linked seven transmembrane domain cytokine receptor. CCR9 serves as a receptor for CCL25/TECK. It is expressed on mature and immature thymocytes and some peripheral T and B cells.

Alternate Names

CCR9, CD199, CDw199

Entrez Gene IDs

10803 (Human); 12769 (Mouse); 282832 (Rat)

Gene Symbol

CCR9

UniProt

P51686

Additional CCR9 Products

Product Documents for Human CCR9 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CCR9 Antibody

For research use only

Citations for Human CCR9 Antibody

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Protocols

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FAQs

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