Human Dkk-1 Antibody

Name: Human Dkk-1 Antibody
Brand: R&D Systems
SKU: MAB10962
Rating: 5 (2 reviews)

Catalog #: MAB10962
5 Citations 2 Reviews Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB10962-SP
MAB10962-500
MAB10962-100

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Product Details

Citations (5)

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Supplemental Products

Reviews (2)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Dkk-1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Dkk-1 in ELISAs. In sandwich immunoassays, no cross-reactivity or interference with recombinant human (rh) Dkk-4, rhKremen-1, rhKremen-2 or rhLRP-6 is observed.

Source

Monoclonal Mouse IgG_2B Clone # 141135

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Dkk-1
Thr32-His266
Accession # O94907

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

8-25 µg/mL

See below

Human Dkk-1 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

2-8 µg/mL

Use in combination with:

Detection Reagent: Human Dkk‑1 Biotinylated Antibody (Catalog # BAF1096)

Standard: Recombinant Human Dkk-1 Protein (Catalog # 5439-DK)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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Dkk‑1 in Human Colon. Dkk-1 was detected in immersion fixed paraffin-embedded sections of human colon using Human Dkk-1 Monoclonal Antibody (Catalog # MAB10962) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002). Specific staining was localized to epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Dkk-1

Members of the dickkopf-related protein family (Dkk-1, -2, -3, and -4) are secreted proteins with two cysteine-rich domains separated by a linker region. Dkk-3 and -4 also have one prokineticin domain. Dkk-1, -2, and -4 function as antagonists of canonical Wnt signaling by binding to LRP5/6, preventing interaction of LRP5/6 with Wnt-Frizzled complexes. Dkk-1, -2, and -4 also bind cell surface Kremen-1 or -2 and promote the internalization of LRP5/6. Antagonistic activity of Dkk-3 has not been demonstrated. Dkk proteins have distinct patterns of expression in adult and embryonic tissues and have a wide range of effects on tissue development and morphogenesis. The Dkk family also includes Soggy, which is homologous to Dkk-3 but not to the other family members. Soggy has not been shown to inhibit Wnt signaling, and its role in the pathway is unclear.

Long Name

Dickkopf-1

Entrez Gene IDs

22943 (Human); 13380 (Mouse); 102123735 (Cynomolgus Monkey)

Alternate Names

dickkopf (Xenopus laevis) homolog 1; dickkopf homolog 1 (Xenopus laevis); dickkopf related protein-1; Dickkopf-1; dickkopf-related protein 1; Dkk1; Dkk-1; hDkk-1; SKdickkopf-1 like

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human Dkk-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Role of Wnt/ beta -catenin and RANKL/OPG in bone healing of diabetic Charcot arthropathy patients
Authors: Agnetha Folestad, Martin Ålund, Susanne Asteberg, Jesper Fowelin, Ylva Aurell, Jan Göthlin et al.
Acta Orthopaedica
Dickkopf-1 is regulated by the mevalonate pathway in breast cancer
Authors: Tilman D Rachner, Andy Göbel, Stefanie Thiele, Martina Rauner, Peggy Benad-Mehner, Peyman Hadji et al.
Breast Cancer Research
Decreased osteogenesis, increased cell senescence and elevated Dickkopf-1 secretion in human fracture non union stromal cells.
Authors: Bajada S, Marshall MJ, Wright KT, Richardson JB, Johnson WE
Bone, 2009-06-18;45(4):726-35.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development
Serum concentrations of DKK-1 decrease in patients with multiple myeloma responding to anti-myeloma treatment.
Authors: Heider U, Kaiser M, Mieth M, Lamottke B, Rademacher J, Jakob C, Braendle E, Stover D, Sezer O
Eur. J. Haematol., 2008-11-10;82(1):31-8.
Species: Human
Sample Types: Serum
Applications: ELISA Development
Myeloma-derived Dickkopf-1 disrupts Wnt-regulated osteoprotegerin and RANKL production by osteoblasts: a potential mechanism underlying osteolytic bone lesions in multiple myeloma.
Authors: Qiang YW, Chen Y, Stephens O, Brown N, Chen B, Epstein J, Barlogie B, Shaughnessy JD
Blood, 2008-02-27;112(1):196-207.
Species: Human, Mouse
Sample Types: Bone Marrow Fluid, Whole Cells
Applications: ELISA Development, Neutralization