Human EpCAM/TROP1 Antibody (158210)

R&D Systems | Catalog # MAB960

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Plant - Brassica Rapa, Plant - Nicotiana benthamiana, Xenograft

Applications

Validated:

Multiplex Immunofluorescence, Immunohistochemistry, Western Blot, Immunocytochemistry, COMET

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Neutralization, Flow Cytometry, Immunocytochemistry, Adhesion Assay, Cell Capture, ELISA Capture, Phagocytosis Assay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 158210
View all EpCAM/TROP1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human EpCAM/TROP-1
Gln24-Lys265
Accession # P16422

Specificity

Detects human EpCAM/TROP-1 in direct ELISAs and Western blots. This antibody detects an epitope found in the extracellular domain between amino acids 136 and 265. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) ALCAM, rhBCAM, rhMCAM, rhNCAM-L1, or recombinant mouse (rm) OCAM is observed. In direct ELISAs, less than 5% cross-reactivity with rmEpCAM/TROP-1 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human EpCAM/TROP1 Antibody (158210)

Detection of EpCAM/TROP-1 in Human Colon via seqIF™ staining on COMET™

EpCAM/TROP-1 was detected in immersion fixed paraffin-embedded sections of human Colon using Mouse Anti-Human EpCAM/TROP-1, Monoclonal Antibody (Catalog # MAB960) at 1ug/mL at 37° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 555 Goat anti-Mouse IgG Secondary Antibody at 1:100 at 37° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR555MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.

Detection of EpCAM/TROP-1 in Human Lung Cancer via seqIF™ staining on COMET™

EpCAM/TROP-1 was detected in immersion fixed paraffin-embedded sections of human Lung Cancer using Mouse Anti-Human EpCAM/TROP-1, Monoclonal Antibody (Catalog # MAB960) at 1ug/mL at 37° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 555 Goat anti-Mouse IgG Secondary Antibody at 1:100 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR555MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.

Detection of EpCAM/TROP-1 in Human Kidney via seqIF™ staining on COMET™

EpCAM/TROP-1 was detected in immersion fixed paraffin-embedded sections of human Kidney using Mouse Anti-Human EpCAM/TROP-1, Monoclonal Antibody (Catalog # MAB960) at 1ug/mL at 37° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 555 Goat anti-Mouse IgG Secondary Antibody at 1:100 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR555MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.
EpCAM/TROP‑1 antibody in HT-29 Human Cell Line by Immunocytochemistry (ICC).

EpCAM/TROP‑1 in HT-29 Human Cell Line.

EpCAM/TROP-1 was detected in immersion fixed HT-29 human colon adenocarcinoma cell line using Mouse Anti-Human EpCAM/TROP-1 Monoclonal Antibody (Catalog # MAB960) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Mouse IgG Secondary Antibody (green; NL009) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
EpCAM/TROP-1 antibody in HT-29 Human Cell Line by Immunocytochemistry (ICC).

EpCAM/TROP‑1 in HT-29 Human Cell Line.

EpCAM/TROP-1 was detected in immersion fixed HT-29 human colon adenocarcinoma cell line using Mouse Anti-Human EpCAM/TROP-1 Monoclonal Antibody (Catalog # MAB960) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
EpCAM/TROP-1 antibody in Human Adenocarcinoma by Immunohistochemistry (IHC-P).

EpCAM/TROP‑1 in Human Adenocarcinoma.

EpCAM/TROP-1 was detected in immersion fixed paraffin-embedded sections of human adenocarcinoma using Mouse Anti-Human EpCAM/TROP-1 Monoclonal Antibody (Catalog # MAB960) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of EpCAM/TROP‑1 in HT‑29 Human Colon Adenocarcinoma Cell Line (Positive) and Daudi Human Burkitt's Lymphoma Cell Line (Negative) Cells.

EpCAM/TROP‑1 was detected in immersion fixed HT‑29 human colon adenocarcinoma cell line (positive) and Daudi human Burkitt's lymphoma cell line (negative) cells using Mouse Anti-Human EpCAM/TROP‑1 Monoclonal Antibody (Catalog # MAB960) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surface and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human EpCAM/TROP1 Antibody (158210)

Application
Recommended Usage

COMET

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed HT‑29 human colon adenocarcinoma cell line (positive) and Daudi human Burkitt's lymphoma cell line (negative) cells 

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human adenocarcinoma

Multiplex Immunofluorescence

3-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human colon

Western Blot

1 µg/mL
Sample: Recombinant Human EpCAM/TROP‑1 Fc Chimera (Catalog # 960-EP)

Reviewed Applications

Read 2 reviews rated 4.5 using MAB960 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.<br/>*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: EpCAM/TROP1

Epithelial Cellular Adhesion Molecule (EpCAM), also known as KS1/4, gp40, GA733-2, 17-1A, and TROP-1, is a 40 kDa transmembrane glycoprotein composed of a 242 amino acid (aa) extracellular domain with two epidermal-growth-factor-like (EGF-like) repeats within the cysteine-rich N-terminal region, a 23 aa transmembrane domain, and a 26 aa cytoplasmic domain. Human and mouse EpCAM share 82% aa sequence identity. In human, EpCAM also shares 49% aa sequence homology with Trop-2/EGP-1. During embryonic development, EpCAM is detected in fetal lung, kidney, liver, pancreas, skin, and germ cells. In adults, human EpCAM is detected in basolateral cell membranes of all simple, pseudo-stratified, and transitional epithelia, but is not detected in normal squamous stratified epithelia, mesenchymal tissue, muscular tissue, neuro-endocrine tissue, or lymphoid tissue (1). EpCAM expression has been found to increase in actively proliferating epithelia tissues and during adult liver regeneration (1, 2). EpCAM expression is also found to increase in human malignant neoplasias, with most carcinoma expressing EpCAM including those of arising from squamousal epithelia (1). EpCAM has been shown function as a homophilic Ca2+ independent adhesion molecule (3). Homophilic adhesion via EpCAM requires the interaction of both EGF-like repeats, with the first EGF-like repeat mediating reciprocal interaction between EpCAM molecules on opposing cells, while the second repeat is involved in lateral interaction of EpCAM. Lateral interaction of EpCAM lead to the formation of dimers and tetramers (4). During homophilic adhesion the cytoplasmic tail of EpCAM interacts with the actin cytoskeleton via a direct association alpha -actinin (5).

References

  1. Balzar, M. et al. (1999) J. Mol. Med. 77:699.
  2. Boer, C.J. et al. (1999) J. Pathol. 188:201.
  3. Litvinow, S.V. et al. (1994) J. Cell Biol. 125:437.
  4. Balzar, M. et al. (2001) Mol. Cell. Biol. 21:2570.
  5. Balzar, M. et al. (1998) Mol. Cell. Biol. 18:4388.

Long Name

Epithelial Cell Adhesion Molecule

Alternate Names

17-1A, CD326, GA733-2, gp40, KS1/4, M4S1, TACSTD1, TROP1

Entrez Gene IDs

4072 (Human); 17075 (Mouse); 171577 (Rat); 102123042 (Cynomolgus Monkey)

Gene Symbol

EPCAM

UniProt

P16422

Additional EpCAM/TROP1 Products

Product Documents for Human EpCAM/TROP1 Antibody (158210)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human EpCAM/TROP1 Antibody (158210)

For research use only

Citations for Human EpCAM/TROP1 Antibody (158210)

Customer Reviews for Human EpCAM/TROP1 Antibody (158210) (2)

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  • Human EpCAM/TROP-1 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Lung tissue
    Species: Human
    Verified Customer | Posted 10/13/2021
    Human EpCAM/TROP1 Antibody (158210) MAB960
  • Human EpCAM/TROP-1 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: NCI-N87 human gastric carcinoma cell line, HT-29 human colon adenocarcinoma cell line and LoVo human colorectal adenocarcinoma cell line
    Species: Human
    Verified Customer | Posted 07/16/2018

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