Cytochrome c Antibody (2B5F8)
R&D Systems | Catalog # MAB898
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Validated:
Human, Equine
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
Cited:
Immunocytochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 2B5F8
Loading...
Product Specifications
Immunogen
Equine Cytochrome c
Specificity
Detects native human Cytochrome c but not mouse Cytochrome c.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Cytochrome c Antibody (2B5F8)
Immunoprecipitation of Human/Mouse Cytochrome c.
Cytochrome c was immunoprecipitated from lysates (5 x 106cells) of Jurkat human acute T cell leukemia and CTLL-2 mouse cytotoxic T cell line following incubation with 5 µg Human/Equine Cytochrome c Monoclonal Antibody (Catalog # MAB898) for 1 hour at 4 ºC. Cytochrome c-antibody complex-es were absorbed using Protein A Immunoprecipitin (Life Tech-nologies). Immunoprecipitated Cytochrome c (left panel) was detected by Western blot using 0.5 µg/mL Human/Mouse/Rat Cytochrome c Monoclonal Antibody (Catalog # MAB897). For additional reference, Western blot (right panel) shows lysates of Jurkat and CTLL2 cell lines probed with 15 µg/mL Human/Mouse/Rat Cytochrome c Monoclonal Antibody (Catalog # MAB897). View our recommended buffer recipes for immunoprecipitation.
Cytochrome c in Human PBMCs.
Cytochrome c was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with ionomycin and PMA using Human/Equine Cytochrome c Monoclonal Antibody (Catalog # MAB898) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Cytochrome c Antibody (2B5F8)
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with ionomycin and PMA
Sample: Immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with ionomycin and PMA
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (basal ganglia)
Sample: Immersion fixed paraffin-embedded sections of human brain (basal ganglia)
Immunoprecipitation
1 µg/106 cells
Sample: Jurkat human acute T cell leukemia cell line and CTLL‑2 mouse cytotoxic T cell line, see our available Western blot detection antibodies
Sample: Jurkat human acute T cell leukemia cell line and CTLL‑2 mouse cytotoxic T cell line, see our available Western blot detection antibodies
Formulation, Preparation, and Storage
Purification
Protein A or G purified from ascites
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Loading...
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Cytochrome c
Additional Cytochrome c Products
Product Documents for Cytochrome c Antibody (2B5F8)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Cytochrome c Antibody (2B5F8)
For research use only
Related Research Areas
Citations for Cytochrome c Antibody (2B5F8)
Powered by Bioz
Customer Reviews for Cytochrome c Antibody (2B5F8)
There are currently no reviews for this product. Be the first to review Cytochrome c Antibody (2B5F8) and earn rewards!
Have you used Cytochrome c Antibody (2B5F8)?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 for a review with an image
$10/€7/£6/$10CAN/¥1110 for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
Loading...
Associated Pathways
