Human FATP1 Antibody (308420)

FATP1 in HeLa Human Cell Line.

FATP1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human FATP1 Monoclonal Antibody (Catalog # MAB3304) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of FATP1 by Western Blot

Linoleic acid (C18) and estradiol stimulate FATP1/SLC27A1 expression and estradiol stimulates the binding of ER-beta to FATP1/SLC27A1 promoter (A) FATP1/SLC27A1, (C) ESR1 and (D) ESR2 expression levels in MDA-MB-231 cells in a pulse chase experiment referenced to the control condition. (B) Western blotting for FATP1 detection, the numbers are indicative of fold change of each condition (normalized for the respective beta -actin) in relation to control. (C) ESR1 and (D) ESR2 expression levels in MDA-MB-231 cells in a pulse chase experiment referenced to the control condition. In relative qPCR, hypoxanthine-guanine phosphoribosyltransferase (HPRT) gene was used as housekeeping gene. (E) Relative occupancy of ER-alpha and ER-beta at FATP1/SLC27A1 promoter. Cells were cultured in control and in estradiol conditions and ER-alpha and ER-beta binding was assessed by ChIP. Data are mean ± error bars of biological triplicates, only adherent cells were analyzed, dead cells in culture media were discarded. (F) Kaplan-Meier survival curves for patients with grade 3, TNBC (n = 77) and grade 3, luminal A BC (n = 75) for the ESR2 gene. *p < 0.05 **p < 0.01 ***p < 0.001. (*) represents the statistical analysis in relation to control condition (dot line). For pulse chase qPCR experiments a Two-way ANOVA with Tukey’s test was used. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31575907), licensed under a CC-BY license. Not internally tested by R&D Systems.