Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 10044
View all FGF basic/FGF2/bFGF Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived human FGF basic/FGF2/bFGF
Accession # P09038

Specificity

Detects human FGF basic/FGF2/bFGF in direct ELISA

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human FGF basic/FGF2/bFGF Antibody

Detection of FGF basic/FGF2/bFGF in A172 cells (positive) and Daudi cells (negative).

FGF basic/FGF2/bFGF was detected in immersion fixed A172 human glioblastoma cells (positive) and absent in Daudi human Burkitt's lymphoma cells (negative) using Mouse Anti-Human FGF basic/FGF2/bFGF Monoclonal Antibody (Catalog # MAB2333) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human FGF basic/FGF2/bFGF Antibody

Application
Recommended Usage

Immunocytochemistry

5-25 µg/mL
Sample: Immersion fixed A172 human glioblastoma cells (positive) and Daudi human Burkitt's lymphoma cells (negative).

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FGF basic/FGF2/bFGF

FGF basic, also known as FGF-2 and HBGF-2, is a member of the FGF family of mitogenic proteins. FGF basic is widely expressed and affects diverse aspects of embryonic development by regulating cell proliferation, differentiation, and survival. Various N-terminal extensions affect localization of FGF basic in cellular compartments but do not affect biological activity. Binding of FGF to heparin or cell surface heparan sulfate proteoglycans is necessary for binding of FGF to high affinity FGF receptors. Mature human FGF basic shares greater than 96% aa sequence identity with bovine, mouse, and rat FGF basic.

Long Name

Fibroblast Growth Factor basic

Alternate Names

bFGF, FGF-2, FGF2, HBGF-2, Prostatropin

Entrez Gene IDs

2247 (Human); 14173 (Mouse); 281161 (Bovine); 403857 (Canine); 100033955 (Equine)

Gene Symbol

FGF2

UniProt

P09038

Additional FGF basic/FGF2/bFGF Products

Product Documents for Human FGF basic/FGF2/bFGF Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human FGF basic/FGF2/bFGF Antibody

For research use only

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