Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot, Flow Cytometry, CyTOF-ready

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Frizzled-6 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Frizzled-6
His19-Val153
Accession # O60353

Specificity

Detects human Frizzled-6 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Frizzled‑6 Antibody

Frizzled-6 antibody in Human Kidney Cancer Tissue by Immunohistochemistry (IHC-P).

Frizzled‑6 in Human Kidney Cancer Tissue.

Frizzled-6 was detected in immersion fixed paraffin-embedded sections of human kidney cancer tissue using Goat Anti-Human Frizzled-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3149) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; (CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cell membranes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Frizzled-6 by Flow Cytometry

Detection of Frizzled-6 by Flow Cytometry

miR-20b and miR-125b augment Wnt signalling through targeting FZD6. (h) sphere formation of MES 83 expressing control shRNA or shRNAs of FZD6, with or without re-expression of FZD6, or a control (right). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/27698350), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Frizzled-6 by Western Blot

Detection of Frizzled-6 by Western Blot

miR-20b and miR-125b augment Wnt signalling through targeting FZD6. (d) IB for FZD6 and APC in miR-transduced MES 83 and 1123, or miRZIP-transduced PN 157 and 84. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/27698350), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Frizzled-6 by Western Blot

Detection of Frizzled-6 by Western Blot

FZD6 mediates induction of the MES-associated phenotype and suppression of the Wnt signalling through TAK1.(a) IB analysis on PN 23 and 17 spheres with or without FZD6 overexpression using indicated antibodies. (b) qRT–PCR analysis of PN-associated genes OLIG2 and SOX2, and MES-associated genes ALDH1A3 and CD44 in PN 23 spheres with or without FZD6 overexpression. (c) FZD6 expression promoted PN 23 spheres proliferation and self-renewal. (d) Ectopic expression of FZD6 promoted tumour growth of PN 23 spheres in GBM xenografts in the brain (n=5). Scale bar, 1.0 mm. (e) TOPFlash/FOPFlash assays on PN 23 with overexpressing FZD6 or control, with or without CaMKII inhibitor KN93 (5 μM), TAK1 inhibitor 5Z-7-oxo (5 μM), or a shRNA-NLK. (f,g) qRT–PCR analyses of the relative expression of miR-20b and miR-125b (f), and Wnt signalling target gene LEF1 (g) in PN 23 overexpressing FZD6, or a vector control, with or without CaMKII inhibitor KN93 (5 μM), TAK1 inhibitor 5Z-7-oxo (5 μM), or a shRNA-NLK. (h) sphere formation assays for PN 23 and 17 spheres with or without overexpression of FZD1, FZD3, and FZD5, respectively. (i) Summary of data shown in Supplementary Fig. 10. An overview of transmembrane receptors and Wnt ligands that affect FZD6 overexpression-induced phenotypes. Data were normalized to the control. *Relative inhibitory effects on indicated protein expression or cellular properties. Error bars (s.d.) represent the data of triplicate samples for each cell line or tumours from five individual mice in each group. *P<0.05, **P<0.01, paired two-way Student's t-test. Data are representative from three independent experiments with similar results. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/27698350), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human Frizzled‑6 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: HEK293 human embryonic kidney cell line

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human kidney cancer tissue

Western Blot

0.1 µg/mL
Sample: Recombinant Human Frizzled‑6

Reviewed Applications

Read 5 reviews rated 4.8 using AF3149 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Frizzled-6

Frizzled-6 is a member of the Frizzled/Smoothened family of G-protein coupled receptors that mediate the Wnt beta-Catenin signaling pathway.

Alternate Names

Frizzled6, FZD6

Entrez Gene IDs

8323 (Human); 14368 (Mouse)

Gene Symbol

FZD6

UniProt

O60353

Additional Frizzled-6 Products

Product Documents for Human Frizzled‑6 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Frizzled‑6 Antibody

For research use only

Related Research Areas

Wnt Family
Wnt Receptors

Citations for Human Frizzled‑6 Antibody

Customer Reviews for Human Frizzled‑6 Antibody (5)

4.8 out of 5
5 Customer Ratings
5 Stars
80%
4 Stars
20%
3 Stars
0%
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0%
1 Stars
0%

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Customer Images

Showing  1 - 5 的 5 reviews Showing All
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  • Human Frizzled-6 Antibody
    Name: Ruchi Shah
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: corneal tissue
    Species: Human
    Verified Customer | Posted 03/21/2023
    1% formalin fixed 0.5% Triton-X100 permeabilized 5% BSA blocking
    Human Frizzled‑6 Antibody AF3149
  • Human Frizzled‑6 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: MDA-MBA-231 human breast cancer cell line
    Species: Human
    Verified Customer | Posted 10/26/2015
    Specificity: Specific<br />Sensitivity: Sensitive<br />Buffer: PBS with 2%BSA<br />Dilution: 1:250
    Human Frizzled‑6 Antibody AF3149
  • Human Frizzled‑6 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: SUM-159PT human breast cancer cell line
    Species: Human
    Verified Customer | Posted 10/26/2015
    4% PFA fixation, Secondary Ab: Alexa488-donkey-anti-goat 1/500. <br />Specificity: Specific<br />Sensitivity: Sensitive<br />Buffer: PBS/Tween<br />Dilution: 1/250
  • Human Frizzled‑6 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: MDA-MB-231 human breast cancer cell line
    Species: Human
    Verified Customer | Posted 10/26/2015
    Cells were fixed in 4% PFA. Secondary Ab: Alexa488-donkey-anti-goat 1/500. <br />Specificity: Specific<br />Sensitivity: Sensitive<br />Buffer: PBS/Tween<br />Dilution: 1/250
  • Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: See PMID 24274766
    Species: Human
    Verified Customer | Posted 01/07/2015

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Protocols

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