Human GM-CSF Antibody (6804)

R&D Systems | Catalog # MAB615

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

ELISA Capture (Matched Antibody Pair), Intracellular Staining by Flow Cytometry, CyTOF-ready

Cited:

Western Blot, Flow Cytometry, ELISA Capture, ELISA Development, Luminex Development

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 6804
View all GM-CSF Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human GM-CSF
Ala18-Glu144
Accession # P04141

Specificity

Detects human GM-CSF in ELISAs. In ELISAs, no cross-reactivity with recombinant mouse GM‑CSF, recombinant rat GM‑CSF, recombinant human (rh) M‑CSF, rhG‑CSF, rhIL‑5‑R alpha, or rhIL‑3 R alpha is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human GM-CSF Antibody (6804)

Detection of GM‑CSF in Human Dendritic Cells by Flow Cytometry.

Human dendritic cells treated with LPS, Recombinant Human TNF-a (210-TA), and Recombinant Human TGF-beta 1 (240-B) were stained with Mouse Anti-Human GM-CSF Monoclonal Antibody (Catalog # MAB615, filled histogram) or isotype control antibody (IC002P, open histogram) followed by Goat anti-Mouse IgG PE-conjugated Secondary Antibody (F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). Staining was performed using our Staining Intracellular Molecules protocol.
Detection of GM-CSF by Western Blot

Detection of GM-CSF by Western Blot

Kinetics of Azithromycin Effects on NALP3 Protein in THP-1 Monocytes. a THP-1 cells were treated with azithromycin 50ug/ml for the times shown prior to immunoblot analysis for indicated proteins. b THP-1 cells were treated with LPS 500 ng/ml for times shown prior to analysis. c THP-1 cells were treated with azithromycin (50ug/ml) and LPS (500 ng/ml) for times shown prior to immunoblot analysis. The data representative of 2–3 separate experiments Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/28659178), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of GM-CSF by Western Blot

Detection of GM-CSF by Western Blot

Kinetics of Azithromycin Effects on NALP3 Protein in THP-1 Monocytes. a THP-1 cells were treated with azithromycin 50ug/ml for the times shown prior to immunoblot analysis for indicated proteins. b THP-1 cells were treated with LPS 500 ng/ml for times shown prior to analysis. c THP-1 cells were treated with azithromycin (50ug/ml) and LPS (500 ng/ml) for times shown prior to immunoblot analysis. The data representative of 2–3 separate experiments Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/28659178), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of GM-CSF by Western Blot

Detection of GM-CSF by Western Blot

Kinetics of Azithromycin Effects on NALP3 Protein in THP-1 Monocytes. a THP-1 cells were treated with azithromycin 50ug/ml for the times shown prior to immunoblot analysis for indicated proteins. b THP-1 cells were treated with LPS 500 ng/ml for times shown prior to analysis. c THP-1 cells were treated with azithromycin (50ug/ml) and LPS (500 ng/ml) for times shown prior to immunoblot analysis. The data representative of 2–3 separate experiments Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/28659178), licensed under a CC-BY license. Not internally tested by R&D Systems.
Human GM-CSF ELISA Standard Curve

Human GM-CSF ELISA Standard Curve

Recombinant Human GM‑CSF (Catalog # 215-GM) was serially diluted and captured by Mouse Anti-Human GM‑CSF Monoclonal Antibody (Catalog # MAB615) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human GM‑CSF Monoclonal Antibody (Catalog # MAB215) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Human GM-CSF Antibody (6804)

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

0.25 µg/106 cells
Sample: Human mature dendritic cells treated with LPS, Recombinant Human TNF‑ alpha (Catalog # 210‑TA), and Recombinant Human IL‑1 beta /IL‑1F2 (Catalog # 201-LB) fixed with paraformaldehyde and permeabilized with saponin

Human GM-CSF Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Human GM‑CSF Biotinylated Antibody (Catalog # BAM215)
  • Standard: Recombinant Human GM-CSF Protein (Catalog # 215-GM)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 4 using MAB615 in the following applications:

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GM-CSF

GM-CSF is a growth factor produced by a variety of lymphoid cells. It binds to receptor heterodimers consisting of a GM-CSF-specific alpha chain and the common beta  chain that is shared by the high affinity receptors for IL-3 and IL-5.

Long Name

Granulocyte Macrophage Growth Factor

Alternate Names

CSF-2, CSF2, GMCSF, Molgramostim, Sargramostim

Entrez Gene IDs

1437 (Human); 12981 (Mouse); 116630 (Rat); 397208 (Porcine); 403923 (Canine); 493805 (Feline)

Gene Symbol

CSF2

UniProt

P04141 (Human)

Additional GM-CSF Products

Product Documents for Human GM-CSF Antibody (6804)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human GM-CSF Antibody (6804)

For research use only

Citations for Human GM-CSF Antibody (6804)

Customer Reviews for Human GM-CSF Antibody (6804) (2)

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Customer Images

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  • Human GM-CSF Antibody
    Name: Anonymous
    Application: Affinity Purification
    Sample Tested: CHO Chinese hamster ovary cell line
    Species: Hamster
    Verified Customer | Posted 12/13/2017
  • Human GM-CSF Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human
    Verified Customer | Posted 11/02/2017
    This antibody was used as a capture in developing a sandwich ELISA for measuring GM-CSF in human serum and plasma. BAM215 paired very well with this antibody.
    Human GM-CSF Antibody (6804) MAB615

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FAQs for Human GM-CSF Antibody (6804)

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  • Q: Do you have a protocol for the isolation and treatment of dendritic cells used for IC flow staining with Human GM-CSF Antibody, catalog #s MAB615 and IC615?

    A: MagCellect Human CD14+ Cell Isolation Kit, catalog # MAGH105, is used to isolate CD14+ monocytes from PBMCs, as the starting population.  Monocytes are cultured in media with rhIL-4 (catalog # 204-IL) at 20 ng/ml and rhGM-CSF (catalog # 215-GM) at 50ng/ml. On day 6, add LPS at 20ng/ml, rhTNF-a (catalog # 210-TA) at 20ng/ml and rhIL-1b (catalog # 201-LB) at 20ng/ml to culture medium and incubate cells overnight. Collect cells for flow cytometric analysis on day 7.

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