Human HLA-DR Alexa Fluor® 405-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB4869V-100
FAB4869V-025
Human HLA-DR Alexa Fluor® 405-conjugated Antibody in Data
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Human HLA-DR Alexa Fluor® 405-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human HLA‑DR.
Source
Monoclonal Mouse IgG1 Clone # L203
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
RPMI 8866 human lymphoblastoid cells
Accession # P01903
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 405 (Excitation= 405 nm, Emission= 421 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
5 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of HLA‑DR in Human PBMCs by Flow Cytometry. View Larger

Detection of HLA‑DR in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD19 PE-conjugated Monoclonal Antibody (Catalog # FAB4867P) and either (A) Mouse Anti-Human HLA-DR Alexa Fluor® 405-conjugated Monoclonal Antibody (Catalog # FAB4869V) or (B) Mouse IgG1Alexa Fluor 405 Isotype Control (Catalog # IC002V). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: HLA-DR

HLA-DR is a human transmembrane major histocompatibility complex 2 (MHC II) family member that consists of a 34 kDa (alpha) subunit and one of several 28 kDa (beta) subunits. HLA-DR is expressed primarily by B cells and dendritic cells (DC) on which it binds peptides derived from internalized and processed antigenic proteins. It presents these peptides on the cell surface for recognition by the T cell receptor on CD4+ T cells. This interaction is central to antigen specificity in the adaptive immune response. HLA-DR alleles, polymorphisms, and aberrant expression are linked to a variety of diseases including autoimmunity and cancer.

Long Name
Major Histocompatibility Complex Class II DR
Entrez Gene IDs
3122 (Human)
Alternate Names
FLJ51114; histocompatibility antigen HLA-DR alpha; HLA class II histocompatibility antigen, DR alpha chain; HLADR; HLA-DR; HLA-DRA; HLA-DRA1; major histocompatibility complex, class II, DR alpha; MHC cell surface glycoprotein; MHC class II antigen DRA; MHC Class II DR; MLRW

Product Datasheets

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