Human HLA-DR Alexa Fluor® 594-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry View Larger

Detection of HLA‑DR in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD19 APC-conjugated Monoclonal Antibody (Catalog # FAB4867A) and either (A) Mouse Anti-Human HLA-DR Alexa Fluor® 594-conjugated Monoclonal Antibody (Catalog # FAB4869T) or (B) Mouse IgG₁Alexa Fluor 594 Isotype Control (Catalog # IC002T). View our protocol for Staining Membrane-associated Proteins.

Preparation and Storage

Background: HLA-DR

HLA-DR is a human transmembrane major histocompatibility complex 2 (MHC II) family member that consists of a 34 kDa (alpha) subunit and one of several 28 kDa (beta) subunits. HLA-DR is expressed primarily by B cells and dendritic cells (DC) on which it binds peptides derived from internalized and processed antigenic proteins. It presents these peptides on the cell surface for recognition by the T cell receptor on CD4⁺ T cells. This interaction is central to antigen specificity in the adaptive immune response. HLA-DR alleles, polymorphisms, and aberrant expression are linked to a variety of diseases including autoimmunity and cancer.

Product Datasheets

Related Research Areas

• B Cell Activation
• B Cell Markers
• Cancer Biomarkers
• Common Macrophage Markers
• Conventional/Classical Dendritic Cells
• Dendritic Cell Co-stimulatory Molecules
• Inflammatory/Monocyte-derived Dendritic Cells
• Langerhans and Dermal Dendritic Cells
• Microglia Activation Markers
• Myeloid-derived Suppressor Cells (MDSC)
• Plasmacytoid Dendritic Cells
• Regulatory T Cells (Tregs)