Human Integrin beta 3/CD61 Antibody (256809)

R&D Systems | Catalog # MAB2266

R&D Systems
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Flow Cytometry, CyTOF-ready

Cited:

Flow Cytometry, ELISA Detection

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 256809
View all Integrin beta 3/CD61 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Integrin beta 3/CD61
Gly27-Asp718
Accession # P05106

Specificity

Detects human Integrin  beta 3/CD61 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human Integrin  beta 1, beta 2, recombinant mouse Integrin beta 4, or beta 6 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human Integrin beta 3/CD61 Antibody (256809)

Detection of Integrin  beta 3/CD61 in PBMC monocytes by Flow Cytometry

PBMC monocytes were stained with Mouse Anti-Human CD14 APC‑conjugated Monoclonal Antibody (Catalog # FAB3832A) and either (A) Mouse Anti-Human Integrin  beta 3/CD61 Monoclonal Antibody (Catalog # MAB2266) or (B) isotype control antibody (Catalog # MAB003) followed by Phycoerythrin-conjugated Anti-Mouse IgM Secondary Antibody (Catalog # F0116). View our protocol for Staining Membrane-associated Proteins.

Applications for Human Integrin beta 3/CD61 Antibody (256809)

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: Human peripheral blood mononuclear cells

Western Blot

1 µg/mL
Sample: Recombinant Human Integrin  beta 3/CD61 under non-reducing conditions only

Reviewed Applications

Read 1 review rated 5 using MAB2266 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.<br/>*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Integrin beta 3/CD61

Integrin beta 3, also called CD61, associates with the Integrin alpha IIb or alpha V subunits to form heterodimeric receptors for collagens, fibronectin, vitronectin, fibrinogen, Von willebrand factor or thrombospondin. alpha IIb/ beta 3, also known as GPIIb IIIa (Glycoprotein IIb-IIIa), is expressed on platelets. alpha V/ beta 3, also known as vitronectin receptor, is expressed on endothelial cells, some B cells, platelets and monocytes.

Alternate Names

CD61, GP3A, GPIIIA, INGRB3, ITGB3

Entrez Gene IDs

3690 (Human); 16416 (Mouse); 29302 (Rat); 102117791 (Cynomolgus Monkey)

Gene Symbol

ITGB3

UniProt

P05106

Additional Integrin beta 3/CD61 Products

Product Documents for Human Integrin beta 3/CD61 Antibody (256809)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Integrin beta 3/CD61 Antibody (256809)

For research use only

Citations for Human Integrin beta 3/CD61 Antibody (256809)

Customer Reviews for Human Integrin beta 3/CD61 Antibody (256809) (1)

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  • Human Integrin  beta 3/CD61 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Serum
    Species: Human
    Verified Customer | Posted 03/23/2022
    Human Integrin beta 3/CD61 Antibody (256809) MAB2266

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