GLP/EHMT1 Antibody (B0422)

R&D Systems | Catalog # PP-B0422-00

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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse, Rat, Transgenic Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Direct ELISA, Immunoprecipitation

Cited:

Immunohistochemistry, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # B0422
View all GLP/EHMT1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant mouse GLP/EHMT1
aa 134-234

Specificity

This antibody specifically recognizes mouse GLP and cross-reacts with human GLP. Not yet tested in other species.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for GLP/EHMT1 Antibody (B0422)

Detection of GLP/EHMT1 by Western Blot

Detection of GLP/EHMT1 by Western Blot

p65 is present in a complex with G9a and GLP under TNF alpha -stimulated conditions in 3T3-L1 adipocytes. Fully differentiated 3T3-L1 adipocytes were treated with 0.75 nM TNF alpha or an equivalent volume of vehicle (0.1% BSA/PBS) for 30 min. (A) Monolayers were collected and subjected to subcellular fractionation. As shown in the left-hand portion of the figure, cytosolic and nuclear protein extracts (300 μg total protein/sample) were immunoprecipitated (IP) with an anti-G9a antibody. The mock sample contained anti-G9a antibody without cell extract and was used as a negative control. (B) The left-hand portion of the figure shows IP of nuclear extract (300 μg total protein/sample) using anti-GLP antibody. The mock sample contained anti-G9a antibody (A) or anti-GLP antibody (B) without protein extract and was used as a negative control in each experiment. The samples on the right-hand portion of (A) or (B) are direct western blot controls containing 25 μg total protein that were analyzed via immunoblotting without IP. This is a representative figure of an experiment independently performed three times. (See Figures S6 and S7 for the original Western blot images). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37237488), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of GLP/EHMT1 by Western Blot

Detection of GLP/EHMT1 by Western Blot

p65 is present in a complex with G9a and GLP under TNF alpha -stimulated conditions in 3T3-L1 adipocytes. Fully differentiated 3T3-L1 adipocytes were treated with 0.75 nM TNF alpha or an equivalent volume of vehicle (0.1% BSA/PBS) for 30 min. (A) Monolayers were collected and subjected to subcellular fractionation. As shown in the left-hand portion of the figure, cytosolic and nuclear protein extracts (300 μg total protein/sample) were immunoprecipitated (IP) with an anti-G9a antibody. The mock sample contained anti-G9a antibody without cell extract and was used as a negative control. (B) The left-hand portion of the figure shows IP of nuclear extract (300 μg total protein/sample) using anti-GLP antibody. The mock sample contained anti-G9a antibody (A) or anti-GLP antibody (B) without protein extract and was used as a negative control in each experiment. The samples on the right-hand portion of (A) or (B) are direct western blot controls containing 25 μg total protein that were analyzed via immunoblotting without IP. This is a representative figure of an experiment independently performed three times. (See Figures S6 and S7 for the original Western blot images). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37237488), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for GLP/EHMT1 Antibody (B0422)

Application
Recommended Usage

Direct ELISA

This antibody can be used at 0.1 μg/mL with the appropriate secondary reagents to detect mouse GLP.

Immunohistochemistry

This antibody can be used at 5 μg/mL with the appropriate secondary reagents to detect mouse GLP.

Immunoprecipitation

Optimal dilutions should be determined by each laboratory.

Western Blot

This antibody can be used at 0.5 μg/mL with the appropriate secondary reagents to detect mouse GLP.

Reviewed Applications

Read 8 reviews rated 4.3 using PP-B0422-00 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

A liquid formulation in physiologic saline with 0.1% NaN<sub>3</sub>.

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

This antibody is stable for greater than six months when held at -20 °C in a manual defrost freezer or at -70 °C. Upon thawing, the antibody can be stored at 2-8 °C for at least 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Background: GLP/EHMT1

Histone H3 Lys 9 (H3-K9) methylation is a crucial epigenetic marker for transcriptional silencing. G9a is the major mammalian H3-K9 methyltransferase that targets euchromatic regions and is essential for murine embryogenesis. There is a single G9a-related methyltransferase in mammals, called GLP/Eu-HMTase1.

Long Name

G9a-like Protein 1/Euchromatic Histone-Lysine N-Methyltransferase 1

Alternate Names

DEL9q34, EHMT1, Eu-HMTase1, KMT1D, Lysine N-methyltransferase 1D

Entrez Gene IDs

79813 (Human); 77683 (Mouse)

Gene Symbol

EHMT1

Additional GLP/EHMT1 Products

Product Documents for GLP/EHMT1 Antibody (B0422)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for GLP/EHMT1 Antibody (B0422)

For research use only

Related Research Areas

Epigenetics
Notch Pathway

Citations for GLP/EHMT1 Antibody (B0422)

Customer Reviews for GLP/EHMT1 Antibody (B0422) (8)

4.3 out of 5
8 Customer Ratings
5 Stars
25%
4 Stars
75%
3 Stars
0%
2 Stars
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1 Stars
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Customer Images

Showing  1 - 5 的 8 reviews Showing All
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  • Human/Mouse GLP/EHMT1 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Brain tissue
    Species: Human
    Verified Customer | Posted 09/07/2021
    GLP/EHMT1 Antibody (B0422) PP-B0422-00
  • Human/Mouse GLP/EHMT1 Antibody
    Name: Anonymous
    Application: Chromatin Immunoprecipitation
    Sample Tested: B cells
    Species: Human
    Verified Customer | Posted 06/26/2018
    ChIP was performed using PP-B0422-00 or isotype IgG (1 ug antibody/ 100 ug Chromatin). Realtime-PCR was performed using primers specific for promoter of gene of interest and the results are expressed as % input.
    GLP/EHMT1 Antibody (B0422) PP-B0422-00
  • Human/Mouse GLP/EHMT1 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: 293T
    Species: Human
    Verified Customer | Posted 06/22/2018
    293T whole cell lysate was immunoblotted with anti GLP anitibody.
    GLP/EHMT1 Antibody (B0422) PP-B0422-00
  • Human/Mouse GLP/EHMT1 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: CCD-1070Sk human foreskin fibroblast cell line
    Species: Human
    Verified Customer | Posted 06/19/2018
    GLP/EHMT1 Antibody (B0422) PP-B0422-00
  • Human/Mouse GLP/EHMT1 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: RL human non-Hodgkin's lymphoma B cell line
    Species: Human
    Verified Customer | Posted 01/04/2018
    GLP/EHMT1 Antibody (B0422) PP-B0422-00
  • Human/Mouse GLP/EHMT1 Antibody
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: Human Dermal Microvascular Endothelial cells - Paraformaldehyde fixed cells
    Species: Human
    Verified Customer | Posted 07/11/2017
    Immunofluorescence staining of paraformaldehyde fixed Human Dermal Microvascular Endothelial cells. PP-B0422-00 used at 1:100 dilution.
    GLP/EHMT1 Antibody (B0422) PP-B0422-00
  • Human/Mouse GLP/EHMT1 Antibody
    Name: Anonymous
    Application: Immunoprecipitation
    Sample Tested: Human Dermal Microvascular Endothelial whole cell lysate
    Species: Human
    Verified Customer | Posted 04/21/2017
    GLP/EHMT1 Antibody (B0422) PP-B0422-00
  • Human/Mouse GLP/EHMT1 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Human Dermal Microvascular Endothelial whole cell lysate
    Species: Human
    Verified Customer | Posted 04/21/2017
    GLP/EHMT1 Antibody (B0422) PP-B0422-00

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Associated Pathways

Notch Signaling Pathways
Notch Signaling Pathway Thumbnail