Human/Mouse/Rat STAT3 Antibody

R&D Systems | Catalog # AF1799

R&D Systems
Discontinued Product
AF1799 has been discontinued. An alternative/replacement product is available: MAB1799. View all STAT3 products.

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse, Rat

Applications

Validated:

Immunocytochemistry, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP)

Cited:

Chromatin Immunoprecipitation (ChIP)

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all STAT3 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse STAT3
Arg688-Met770
Accession # U06922

Specificity

Detects human, mouse, and rat STAT3 from whole cell extracts.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat STAT3 Antibody

Detection of STAT3-regulated Genes antibody by Chromatin Immunoprecipitation.

Detection of STAT3-regulated Genes by Chromatin Immunoprecipitation.

Jurkat human acute T cell leukemia cell line treated with 1000 U/mL Recombinant Human IFN-a Universal Type I (Catalog # 11200-1) for 1 hour was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. STAT3/DNA complexes were immunoprecipitated using 5 µg Goat Anti-Human/Mouse/Rat STAT3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1799) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 µL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. Thec-mycpromoter was detected by standard PCR.
Immunoprecipitation of Human STAT3.

Immunoprecipitation of Human STAT3.

STAT3 was immunoprecipitated from 500 µg of Daudi human Burkitt's lymphoma cell line cytoplasmic and nuclear lystates following incubation with 2 µg Goat Anti-Human/Mouse/Rat STAT3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1799) for overnight at 4 ºC. STAT3-antibody complexes were absorbed using Protein G agarose (Invitrogen). Immunoprecipitated STAT3 was detected by Western blot using 0.1 µg/mL Human STAT3 Monoclonal Antibody (Catalog # MAB1799). View our recommended buffer recipes for immunoprecipitation.
STAT3 antibody in D3 Mouse Cell Line by Immunocytochemistry (ICC).

STAT3 in D3 Mouse Cell Line.

STAT3 was detected in immersion fixed D3 mouse embryonic stem cell line using Goat Anti-Human/Mouse/Rat STAT3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1799) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001). Tubulin was also detected using Tubulin Antibody (Catalog # NB600-506; Novus Biologicals) and stained using the NorthernLights™ 493-conjugated Anti-Rat IgG Secondary Antibody (green; Catalog # NL015). Cells were counterstained with DAPI (blue). Specific staining of STAT3 was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Applications for Human/Mouse/Rat STAT3 Antibody

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

5 µg/5 x 106 cells
Sample: Human IFN‑ alpha  treated Jurkat human acute T cell leukemia cell line chromatin, c-myc promoter detected by standard PCR.

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed mouse D3 embryonic stem cells

Immunoprecipitation

2 µg/500 µg cell lysate
Sample: Daudi human Burkitt's lymphoma cell line, see our available Western blot detection antibodies

Reviewed Applications

Read 1 review rated 5 using AF1799 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: STAT3

The STAT family of transcription factors, first described as key mediators of interferon signal transduction, are now recognized as signaling factors downstream of many cytokines, growth factors, and hormones. At least seven STATs exist: STAT1, 2, 3, 4, 5a, 5b, and 6. They are responsible for an array of cellular activities including regulating growth, survival, differentiation, motility, and the immune response. They are characterized structurally by the presence of the Src homology 2 (SH2) domain, and a C-terminal tyrosine phosphorylation site. Upon ligand binding, they are recruited to the activated receptor, via their SH2 domains, to receptor-associated tyrosine kinases of the Janus kinase family (JAK). Once phosphorylated by JAK, STATS are free to hetero- or homo-dimerize, and translocate to the nucleus where they bind DNA to regulate gene transcription.

Long Name

Signal Transducer and Activator of Transcription 3

Alternate Names

Acute-phase response factor, APRFMGC16063, DNA-binding protein APRF, FLJ20882, HIES, signal transducer and activator of transcription 3, signal transducer and activator of transcription 3 (acute-phase responsefactor)

Entrez Gene IDs

6774 (Human); 20848 (Mouse); 25125 (Rat)

Gene Symbol

STAT3

UniProt

U06922

Additional STAT3 Products

Product Documents for Human/Mouse/Rat STAT3 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat STAT3 Antibody

For research use only

Citations for Human/Mouse/Rat STAT3 Antibody

Customer Reviews for Human/Mouse/Rat STAT3 Antibody (1)

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  • Human/Mouse/Rat STAT3 Antibody
    Name: Dharmaraja Allimuthu
    Application: Immunoprecipitation
    Sample Tested: IPS2 induced pluripotent stem cells
    Species: Human
    Verified Customer | Posted 01/17/2018
    Human/Mouse/Rat STAT3 Antibody AF1799

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