Human/Mouse/Rat STAT3 Antibody

Catalog # Availability Size / Price Qty
MAB1799
MAB1799-SP
Detection of Human STAT3 by Western Blot.
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Product Details
Citations (10)
FAQs
Supplemental Products
Reviews (1)

Human/Mouse/Rat STAT3 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat STAT3 in Western blots.
Source
Monoclonal Mouse IgG2B Clone # 232209
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human STAT3
Met1-Asn175
Accession # P40763
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Complete Your Experiment

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
See below
Immunoprecipitation
1-3 µg/500 µg cell lysate
Daudi human Burkitt's lymphoma cell line, see our available Western blot detection antibodies
Immunocytochemistry
3-25 µg/mL
See below
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
See below
Knockout Validated

STAT3 is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in STAT3 knockout HeLa cell line.

 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human STAT3 by Western Blot. View Larger

Detection of Human STAT3 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, HeLa human cervical epithelial carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human STAT3 Monoclonal Antibody (Catalog # MAB1799) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for STAT3 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry STAT3 in HeLa Human Cell Line. View Larger

STAT3 in HeLa Human Cell Line. STAT3 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human/Mouse/Rat STAT3 Monoclonal Antibody (Catalog # MAB1799) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Intracellular Staining by Flow Cytometry Detection of STAT3 in Jurkat Human Cell Line by Flow Cytometry. View Larger

Detection of STAT3 in Jurkat Human Cell Line by Flow Cytometry. Jurkat human acute T cell leukemia cell line was stained with Mouse Anti-Human STAT3 Monoclonal Antibody (Catalog # MAB1799, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with methanol.

Intracellular Staining by Flow Cytometry Detection of STAT3 in HeLa Human Cell Line by Flow Cytometry. View Larger

Detection of STAT3 in HeLa Human Cell Line by Flow Cytometry. HeLa human cell line was stained with Mouse Anti-Human/Mouse/Rat STAT3 Monoclonal Antibody (Catalog # MAB1799, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram) followed by anti-Mouse IgG PE-conjugated secondary antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Knockout Validated STAT3 Specificity is Shown by Immunocytochemistry in Knockout Cell Line. View Larger

STAT3 Specificity is Shown by Immunocytochemistry in Knockout Cell Line. STAT3 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line treated with IFN-alpha 1, but is not detected in STAT3 knockout (KO) HeLa cell line using Mouse Anti-Human/ Mouse/Rat STAT3 Monoclonal Antibody (Catalog # MAB1799) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Knockout Validated Western Blot Shows Human STAT3 Specificity by Using Knockout Cell Line. View Larger

Western Blot Shows Human STAT3 Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line, STAT1 knockout (KO) HeLa cell line, STAT2 KO HeLa cell line, STAT3 KO HeLa cell line, STAT5a KO HeLa cell line, STAT5b KO HeLa cell line, STAT6 KO HeLa cell line,. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat STAT3 Monoclonal Antibody (Catalog # MAB1799) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for STAT3 at approximately 80 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the STAT3 knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Knockout Validated STAT3 Specificity is Shown by Flow Cytometry in Knockout Cell Line. View Larger

STAT3 Specificity is Shown by Flow Cytometry in Knockout Cell Line. STAT3 knockout HeLa human cervical epithelial cell line was stained with Mouse Anti-Human/Mouse STAT3 Monoclonal Antibody (Catalog # MAB1799, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram) followed by anti-Mouse IgG PE-conjugated secondary antibody (Catalog # F0102B). No staining in the STAT3 knockout HeLa cell line was observed. To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: STAT3

Signal Transducer and Activator of Transcription (STAT) proteins are transcription factors activated in response to cytokine, growth factor, or hormone receptor signaling. Janus kinases (JAKs) phosphorylate STAT proteins and induce dimerization. Homo- or heterodimers translocate to the nucleus where they bind to DNA and activate transcription.

Long Name
Signal Transducer and Activator of Transcription 3
Entrez Gene IDs
6774 (Human); 20848 (Mouse); 25125 (Rat)
Alternate Names
Acute-phase response factor; APRFMGC16063; DNA-binding protein APRF; FLJ20882; HIES; signal transducer and activator of transcription 3 (acute-phase responsefactor); signal transducer and activator of transcription 3; STAT3

Product Datasheets

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Citations for Human/Mouse/Rat STAT3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. Piceatannol, a Dietary Polyphenol, Alleviates Adipose Tissue Loss in Pre-Clinical Model of Cancer-Associated Cachexia via Lipolysis Inhibition
    Authors: JC Kershaw, BD Elzey, XX Guo, KH Kim
    Nutrients, 2022;14(11):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Bacterial Antigens Reduced the Inhibition Effect of Capsaicin on Cal 27 Oral Cancer Cell Proliferation
    Authors: R Chakrabort, K Vickery, C Darido, S Ranganatha, H Hu
    International Journal of Molecular Sciences, 2021;22(16):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Kurarinone Attenuates Collagen-Induced Arthritis in Mice by Inhibiting Th1/Th17 Cell Responses and Oxidative Stress
    Authors: KT Tang, CC Lin, SC Lin, JH Wang, SW Tsai
    International Journal of Molecular Sciences, 2021;22(8):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. ML218 HCl Is More Efficient Than Capsaicin in Inhibiting Bacterial Antigen-Induced Cal 27 Oral Cancer Cell Proliferation
    Authors: R Chakrabort, H Hu, C Darido, K Vickery, S Ranganatha
    International Journal of Molecular Sciences, 2021;22(22):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Opa interacting protein 5 promotes metastasis of nasopharyngeal carcinoma cells by promoting EMT via modulation of JAK2/STAT3 signal
    Authors: YQ Zheng, YR Cui, S Yang, YP Wang, YJ Qiu, WL Hu
    Eur Rev Med Pharmacol Sci, 2019;23(2):613-621.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Circular RNA circHIPK3 modulates autophagy via MIR124-3p-STAT3-PRKAA/AMPK? signaling in STK11 mutant lung cancer
    Authors: X Chen, R Mao, W Su, X Yang, Q Geng, C Guo, Z Wang, J Wang, LA Kresty, DG Beer, AC Chang, G Chen
    Autophagy, 2019;0(0):1-13.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. Tumor-derived mesenchymal-stem-cell-secreted IL-6 enhances resistance to cisplatin via the STAT3 pathway in breast cancer
    Authors: H Xu, Y Zhou, W Li, B Zhang, H Zhang, S Zhao, P Zheng, H Wu, J Yang
    Oncol Lett, 2018;15(6):9142-9150.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. Inhibition of STAT3 Signaling Reduces IgA1 Autoantigen Production in IgA Nephropathy
    Authors: K Yamada, ZQ Huang, M Raska, C Reily, JC Anderson, H Suzuki, H Ueda, Z Moldoveanu, K Kiryluk, Y Suzuki, RJ Wyatt, Y Tomino, AG Gharavi, A Weinmann, BA Julian, CD Willey, J Novak
    Kidney Int Rep, 2017;2(6):1194-1207.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. Proangiogenic tumor proteins as potential predictive or prognostic biomarkers for bevacizumab therapy in metastatic colorectal cancer.
    Authors: Bruhn M, Townsend A, Khoon Lee C, Shivasami A, Price T, Wrin J, Arentz G, Tebbutt N, Hocking C, Cunningham D, Hardingham J
    Int J Cancer, 2014;135(3):731-41.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  10. IL-6 cytoprotection in hyperoxic acute lung injury occurs via suppressor of cytokine signaling-1-induced apoptosis signal-regulating kinase-1 degradation.
    Authors: Kolliputi N, Waxman AB
    Am. J. Respir. Cell Mol. Biol., 2009;40(3):314-24.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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Human/Mouse/Rat STAT3 Antibody
By Anonymous on 08/27/2021
Application: ICC/IF Sample Tested: Hepatocellular carcinoma cell line Species: Human