Human Oct-4A Antibody (653108)

R&D Systems | Catalog # MAB17591

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Intracellular Staining by Flow Cytometry, Immunocytochemistry, CyTOF-ready

Cited:

Western Blot, Flow Cytometry, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 653108
View all Oct-4A Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Oct-4A
Met1-Glu135
Accession # NP_002692

Specificity

Detects human Oct-4A in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human Oct-4A Antibody (653108)

Detection of Oct‑4A in NTERA-2 cells by Flow Cytometry

NTERA-2 cells were stained with Mouse Anti-Human Oct‑4A Monoclonal Antibody (Catalog # MAB17591, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.
Detection of Human Oct-4A antibody by Western Blot.

Detection of Human Oct‑4A by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and NTera-2 human testicular embryonic carcinoma cell line. PVDF Membrane was probed with 0.5 µg/mL of Mouse Anti-Human Oct-4A Monoclonal Antibody (Catalog # MAB17591) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF007). A specific band was detected for Oct-4A at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Oct-4A and E-Cadherin antibody in BG01V Human Stem Cells by Immunocytochemistry (ICC).

Oct-4A and E-Cadherin in BG01V Human Stem Cells.

Oct-4A and E-Cadherin were detected in human BG01V embryonic stem cells grown on irradiated MEF cells using 10 µg/mL Human Oct-4A Monoclonal Antibody (Catalog # MAB17591) and 10 µg/mL Human E-Cadherin Affinity-purified Polyclonal Antibody (AF648). Cells were incubated with primary antibodies for 3 hours at room temperature. Cells were stained for Oct-4A using the Northern-Lights™ 493-conjugated Anti-Mouse IgG Secondary Antibody (green; NL009), and stained for E-Cadherin using the Northern-Lights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001). Specific staining of Oct-4A was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Oct-4A antibody in BG01V Human Stem Cells antibody by Flow Cytometry.

Detection of Oct‑4A in BG01V Human Stem Cells by Flow Cytometry.

BG01V human embryonic stem cells were stained with Human Oct-4A Monoclonal Antibody (Catalog # MAB17591, filled histogram) or isotype control antibody (MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Detection of Oct‑4A in iPSCs by Flow Cytometry.

iPSCs were stained with Mouse Anti-Human Oct‑4A Monoclonal Antibody (Catalog # MAB17591, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Human Oct-4A Antibody (653108)

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed BG01V human embryonic stem cells and immersion fixed ADLF1 and FAB2 induced pluripotent stem cell lines

Intracellular Staining by Flow Cytometry

0.25 µg/106 cells
Sample: NTERA-2 human testicular embryonic carcinoma cell line or BG01V human embryonic stem cells or iPSCs fixed with paraformaldehyde and permeabilized with saponin

Western Blot

0.5 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line and NTera‑2 human testicular embryonic carcinoma cell line

Reviewed Applications

Read 2 reviews rated 4 using MAB17591 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.<br/>*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Oct-4A

Oct-3/4, alternately Oct-3 or Oct-4, is POU5F1 (POU domain containing, class 5, transcription factor 1), a 360 amino acid (aa) transcription factor that is expressed in totipotent embryonic stem and germ cells. The human Oct-4, Oct-3/4 or POU5F1 gene can be transcribed into at least three transcripts (Oct-4A, Oct-4B, and Oct-4B1) and generates four protein isoforms by alternative splicing and alternative translation initiation. Oct-4A expression is restricted to embryonic stem (ES) and embryonic carcinoma (EC) cells and is believed to be the transcription factor responsible for the pluripotency properties of embryonic stem (ES) cells. In contrast, Oct-4B/4B1 can be detected in various nonpluripotent cell types and cannot sustain ES cell pluripotency and self-renewal.

Long Name

Octamer-binding Transcription Factor 4A

Alternate Names

Oct4A

Entrez Gene IDs

5460 (Human); 18999 (Mouse)

Gene Symbol

POU5F1

UniProt

NP_002692

Additional Oct-4A Products

Product Documents for Human Oct-4A Antibody (653108)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Oct-4A Antibody (653108)

For research use only

Citations for Human Oct-4A Antibody (653108)

Customer Reviews for Human Oct-4A Antibody (653108) (2)

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  • Human Oct-4A Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: NTera-2 human testicular embryonic carcinoma cell line
    Species: Human
    Verified Customer | Posted 03/12/2022
    Human Oct-4A Antibody (653108) MAB17591
  • Human Oct-4A Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: WS-1 human fetal skin fibroblast cell line
    Species: Human
    Verified Customer | Posted 01/31/2019
    Human Oct-4A Antibody (653108) MAB17591

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