Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 1058613
View all P2X7/P2RX7 Primary Antibodies »

Product Specifications

Immunogen

Human P2X7/P2RX7 synthetic peptide
Accession # Q99572

Specificity

Detects human P2X7/P2RX7 in direct ELISA.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human P2X7/P2RX7 Antibody

Detection of P2X7/P2RX7 in Hek 293 cells transfected with Human P2RX7 peptide and eGFP by Flow Cytometry.

Hek293 cells transfected with Human P2RX7 peptide and eGFP were stained with either (A) Mouse Anti-Human P2X7/P2RX7 Monoclonal Antibody (Catalog # MAB97903) or (B) Mouse IgG2B Isotype Control (Catalog # MAB004) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Applications for Human P2X7/P2RX7 Antibody

Application
Recommended Usage

Flow Cytometry

0.25 µg/106 cells
Sample: Hek 293 cells transfected with Human P2RX7 peptide and eGFP

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: P2X7/P2RX7

Human P2X purinoceptor 7 (aka P2X7) is a 595 aminoacids (aa) protein encoded by the P2RX7 gene. P2X7 receptors belong to the family of ATP-gated ion channels, and their activity can be found in cells of hemopoietic lineage including macrophages, microglia, and certain lymphocytes. They mediate the influx of Ca2+ and Na+ and the release of pro-inflammatory cytokines. At least 8 different human P2X7 isoforms formed by alternative splicing have been reported, ranging from 128 aa to 595 aa. The P2X7 subunits can form homomeric receptors only with a typical P2X receptor structure.

Long Name

P2X Purinergic Receptor 7

Alternate Names

P2RX7

Entrez Gene IDs

5027 (Human); 18439 (Mouse); 29665 (Rat)

Gene Symbol

P2RX7

UniProt

Q99572

Additional P2X7/P2RX7 Products

Product Documents for Human P2X7/P2RX7 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human P2X7/P2RX7 Antibody

For research use only

Customer Reviews for Human P2X7/P2RX7 Antibody

There are currently no reviews for this product. Be the first to review Human P2X7/P2RX7 Antibody and earn rewards!

Have you used Human P2X7/P2RX7 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies