IL-2 Antibody [Biotin]

R&D Systems | Catalog # BAF202

R&D Systems
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Key Product Details

Species Reactivity

Validated:

Human, Primate

Cited:

Human, Mouse, Primate - Papio anubis (Olive Baboon)

Applications

Validated:

Western Blot, ELISA Detection (Matched Antibody Pair), Immunocytochemistry

Cited:

ELISA Capture, ELISA Detection, ELISA Development, ELISA Development (Detection), ICC/F, Luminex Development

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all IL-2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human IL-2
Ala21-Thr153
Accession # P60568

Specificity

Detects human and primate IL-2 in ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for IL-2 Antibody [Biotin]

IL‑2 antibody in Human PBMCs by Immunocytochemistry (ICC).

IL‑2 in Human PBMCs.

IL‑2 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with PMA and ionomycin using 15 µg/mL Goat Anti-Human/Primate IL‑2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF202) for 3 hours at room temperature. Cells were stained (red) and counterstained (green). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
IL‑2 antibody in Human PBMCs by Immunocytochemistry (ICC).

IL‑2 in Human PBMCs.

IL-2 was detected in immersion fixed PMA-, ionomycin-, and monensin-stimulated human peripheral blood mononuclear cells (PBMCs) using 10 µg/mL Goat Anti-Human/Primate IL-2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF202) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Human IL-2 ELISA Standard Curve

Human IL-2 ELISA Standard Curve

Recombinant Human IL‑2 (Catalog # 202-IL) was serially diluted and captured by Mouse Anti-Human/Primate IL‑2 Monoclonal Antibody (Catalog # MAB602) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-202-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for IL-2 Antibody [Biotin]

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with PMA and ionomycin

Western Blot

0.1 µg/mL
Sample: Recombinant Human IL‑2 (Catalog # 202-IL)

Human/Primate IL-2 Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)
Recommended Concentration: 0.1-0.4 µg/mL
Use in combination with these reagents:
  • Capture Reagent: Human/Primate IL‑2 Antibody (Catalog # MAB602)
  • Standard: Recombinant Human IL-2 Protein (Catalog # 202-IL)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 4 using BAF202 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-2

Interleukin-2 (IL-2) is a O-glycosylated, four alpha -helix bundle cytokine that has potent stimulatory activity for antigen-activated T cells. It is expressed by CD4+ and CD8+ T cells, gamma delta T cells, B cells, dendritic cells, and eosinophils (1 - 3). Mature human IL-2 shares 56% and 66% aa sequence identity with mouse and rat IL-2, respectively. Human and mouse IL-2 exhibit cross-species activity (4). The receptor for IL-2 consists of three subunits that are present on the cell surface in varying preformed complexes (5 - 7). The 55 kDa IL-2 R alpha is specific for IL-2 and binds with low affinity. The 75 kDa IL-2 R beta, which is also a component of the IL-15 receptor, binds IL-2 with intermediate affinity. The 64 kDa common gamma chain gamma c/IL-2 R gamma, which is shared with the receptors for IL-4, -7, -9, -15, and -21, does not independently interact with IL-2. Upon ligand binding, signal transduction is performed by both IL-2 R beta and gamma c. IL-2 is best known for its autocrine and paracrine activity on T cells. It drives resting T cells to proliferate and induces IL-2 and IL-2 R alpha synthesis (1, 2). It contributes to T cell homeostasis by promoting the Fas-induced death of naïve CD4+ T cells but not activated CD4+ memory lymphocytes (8). IL-2 plays a central role in the expansion and maintenance of regulatory T cells, although it inhibits the development of Th17 polarized cells (9 - 11). Thus, IL-2 may be a key cytokine in the natural suppression of autoimmunity (12, 13).

References

  1. Ma, A. et al. (2006) Annu. Rev. Immunol. 24:657.
  2. Gaffen, S.L. and K.D. Liu (2004) Cytokine 28:109.
  3. Taniguchi, T. et al. (1983) Nature 302:305.
  4. Mosmann, T.R. et al. (1987) J. Immunol. 138:1813.
  5. Liparoto, S.F. et al. (2002) Biochemistry 41:2543.
  6. Wang, X. et al. (2005) Science 310:1159.
  7. Bodnar, A. et al. (2008) Immunol. Lett. 116:117.
  8. Jaleco, S. et al. (2003) J. Immunol. 171:61.
  9. Malek, T.R. (2003) J. Leukoc. Biol. 74:961.
  10. Laurence, A. et al. (2007) Immunity 26:371.
  11. Kryczek, I. et al. (2007) J. Immunol. 178:6730.
  12. Afzali, B. et al. (2007) Clin. Exp. Immunol. 148:32.
  13. Fehervari, Z. et al. (2006) Trends Immunol. 27:109.

Long Name

Interleukin 2

Alternate Names

Aldesleukin, IL2, Proleukin, TCGF

Entrez Gene IDs

3558 (Human); 16183 (Mouse); 116562 (Rat); 396868 (Porcine); 280822 (Bovine); 403989 (Canine); 100034204 (Equine); 751114 (Feline); 100302458 (Rabbit)

Gene Symbol

IL2

UniProt

P60568

Additional IL-2 Products

Product Documents for IL-2 Antibody [Biotin]

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for IL-2 Antibody [Biotin]

For research use only

Citations for IL-2 Antibody [Biotin]

Customer Reviews for IL-2 Antibody [Biotin] (2)

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  • Human/Primate IL-2 Biotinylated Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Recombinant protein
    Species: Human
    Verified Customer | Posted 09/17/2019
    IL-2 Antibody [Biotin] BAF202
  • Name: Anonymous
    Application: ELISA
    Sample Tested: See PMID 22059993
    Species: Human
    Verified Customer | Posted 01/12/2015

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