Human RelB Antibody

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RelB in Human Lymphoma.
3 Images
Product Details
Citations (1)
Supplemental Products
Reviews (3)

Human RelB Antibody Summary

Species Reactivity
Detects human RelB.
Monoclonal Mouse IgG2B Clone # 315206
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human RelB
Accession # Q01201
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.1 µg/mL
See below
8-25 µg/mL
See below
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry RelB antibody in Human Lymphoma by Immunohistochemistry (IHC-P). View Larger

RelB in Human Lymphoma. RelB was detected in immersion fixed paraffin-embedded sections of human lymphoma using 8 µg/mL Mouse Anti-Human RelB Monoclonal Antibody (Catalog # MAB2698) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm in epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Western Blot Detection of Human RelB antibody by Western Blot. View Larger

Detection of Human RelB by Western Blot. Western blot shows lysates of Daudi human Burkitt's lymphoma cell line and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human RelB Monoclonal Antibody (Catalog # MAB2698) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for RelB at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.

Immunocytochemistry RelB antibody in Raji Human Cell Line by Immunocytochemistry (ICC). View Larger

RelB in Raji Human Cell Line. RelB was detected in immersion fixed Raji human Burkitt's lymphoma cell line using Mouse Anti-Human RelB Monoclonal Antibody (Catalog # MAB2698) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: RelB

RelB is a member of the NFkB family and can act as either an activator or repressor of transcription by forming heterodimers with the p50 and p52 NFkB family members. Although RelB knock out mice are viable, there are complex abnormalities in their inflammatory response, hematopoietic lineage and formation of secondary lymphoid structures.

Long Name
v-rel Reticuloendotheliosis Viral Oncogene Homolog B
Entrez Gene IDs
5971 (Human)
Alternate Names
IREL; I-Rel; nuclear factor of kappalight polypeptide gene enhancer in B-cells 3; RelB; v-rel reticuloendotheliosis viral oncogene homolog B

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Citation for Human RelB Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Kinome-wide functional genomics screen reveals a novel mechanism of TNFα-induced nuclear accumulation of the HIF-1α transcription factor in cancer cells.
    Authors: Schoolmeesters A, Brown DD, Fedorov Y
    PLoS ONE, 2012-02-15;7(2):e31270.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC


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Reviews for Human RelB Antibody

Average Rating: 3.7 (Based on 3 Reviews)

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Human RelB Antibody
By Anonymous on 03/11/2019
Application: Microarrays Sample Tested: EDTA Plasma Species: Human

Human RelB Antibody
By Anonymous on 02/21/2019
Application: MiAr Sample Tested: EDTA Plasma Species: Human

Human RelB Antibody
By Anonymous on 11/10/2018
Application: ELISA Sample Tested: Plasma Species: Human