Human Smad5 Alexa Fluor® 350-conjugated Antibody

Catalog #: AF2250U Datasheet / COA / SDS

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AF2250U-100UG

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Human Smad5 Alexa Fluor® 350-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Smad5 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) Smad1, rhSmad2 and rhSmad4 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Smad5
Val155-Arg258
Accession # Q99717

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 350 (Excitation= 346 nm, Emission= 442 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Smad5

Smad5 (Smad; an amalgam of the Sma gene in worm and the MAD gene in fruitfly) is a transcriptional modulator that is activated by BMP receptor kinases. Human Smad5 shares 99% amino acid sequence homology with the mouse or rat protein.

Long Name

Mothers Against DPP Homolog 5

Entrez Gene IDs

4090 (Human)

Alternate Names

Dwfc; hSmad5; JV5-1; JV5-1DKFZp781O1323; MAD homolog 5; MAD, mothers against decapentaplegic homolog 5 (Drosophila); MADH5; MADH5mothers against decapentaplegic homolog 5; mothers against decapentaplegic homolog 5; mothers against decapentaplegic, drosophila, homolog of, 5; Mothers against DPP homolog 5; SMA- and MAD-related protein 5; SMAD 5; SMAD family member 5DKFZp781C1895; SMAD, mothers against DPP homolog 5 (Drosophila); SMAD, mothers against DPP homolog 5; Smad5