Human Smad5 Alexa Fluor® 488-conjugated Antibody

Catalog #: AF2250G Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF2250G-100UG

Save 15% on Select RUO Reagents. See Details.

All Smad5 Antibodies

Product Details

Supplemental Products

Reviews

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Smad5 Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Smad5 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) Smad1, rhSmad2 and rhSmad4 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Smad5
Val155-Arg258
Accession # Q99717

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Smad5

Smad5 (Smad; an amalgam of the Sma gene in worm and the MAD gene in fruitfly) is a transcriptional modulator that is activated by BMP receptor kinases. Human Smad5 shares 99% amino acid sequence homology with the mouse or rat protein.

Long Name

Mothers Against DPP Homolog 5

Entrez Gene IDs

4090 (Human)

Alternate Names

Dwfc; hSmad5; JV5-1; JV5-1DKFZp781O1323; MAD homolog 5; MAD, mothers against decapentaplegic homolog 5 (Drosophila); MADH5; MADH5mothers against decapentaplegic homolog 5; mothers against decapentaplegic homolog 5; mothers against decapentaplegic, drosophila, homolog of, 5; Mothers against DPP homolog 5; SMA- and MAD-related protein 5; SMAD 5; SMAD family member 5DKFZp781C1895; SMAD, mothers against DPP homolog 5 (Drosophila); SMAD, mothers against DPP homolog 5; Smad5