Human SOX9 Antibody

Catalog #: MAB3075 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB3075-100
MAB3075-SP

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Detection of SOX9 in U‑251 MG cells (positive) and HEL 92.1.7 cells (negative).

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human SOX9 Antibody Summary

Species Reactivity

Human

Specificity

Detects human SOX9 in direct ELISA.

Source

Monoclonal Mouse IgG_2A Clone # 326820

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human SOX9
Met1-Lys151
Accession # P48436

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunocytochemistry

8-25 µg/mL

Immersion fixed U‑251 MG human glioblastoma cells (positive) and absent in HEL 92.1.7 human erythroleukemic cells (negative)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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Detection of SOX9 in U‑251 MG cells (positive) and HEL 92.1.7 cells (negative). SOX9 was detected in immersion fixed U‑251 MG human glioblastoma cells (positive) and absent in HEL 92.1.7 human erythroleukemic cells (negative) using Mouse Anti-Human SOX9 Monoclonal Antibody (Catalog # MAB3075) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunocytochemistry/ Immunofluorescence

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Immunofluorescent Staining of iPSC-derived Pancreatic Duct-like Organoids. iPSC-derived pancreatic duct-like organoids were fixed and stained with Mouse Anti-Human SOX9 Monoclonal Antibody (Catalog # MAB3075; green) and a Rat Anti-Human Claudin-1 Monoclonal Antibody (Catalog # MAB4618; red) followed by secondary antibody staining with the NorthernLights™ 493-conjugated Donkey Anti-Mouse IgG Antigen Affinity-purified Polyclonal Antibody (Catalog # NL009) and NorthernLights 557-conjugated Goat Anti-Rat IgG Antigen Affinity-purified Polyclonal Antibody (Catalog # NL013). Cell nuclei were stained with DAPI (Catalog # 5748; blue) and the images were overlaid. Images were taken using a Leica THUNDER Imaging System (scale bar: 20 mm).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SOX9

SOX9 belongs to the SOX (SRY-like HMG box) family of transcription factors with diverse roles in development. SOX9 is expressed in mesenchymal progenitors that give rise to chondrocytes and osteoblasts. It is also expressed in the central nervous system, neural crest, intestine, pancreas, and testis. Mutations in SOX9 are associated with defects in sex determination, cartilage and bone development, as well as abnormalities of the heart, kidneys, brain, gut, and pancreas.

Long Name

Transcription Factor SOX9

Entrez Gene IDs

6662 (Human)

Alternate Names

campomelic dysplasia, autosomal sex-reversal; CMD 1; CMD1; CMPD1; SOX9; SRA1SRY (sex-determining region Y)-box 9 protein; SRY (sex determining region Y)-box 9; SRY-related HMG-box, gene 9; transcription factor SOX-9