Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Flow Cytometry

Cited:

Western Blot

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all Survivin Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Survivin

Specificity

Detects human Survivin in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human Survivin Biotinylated Antibody

Application
Recommended Usage

Flow Cytometry

2.5 µg/106 cells
Sample: Human peripheral blood mononuclear cells

Western Blot

0.1 µg/mL
Sample: Recombinant Human Survivin Western Blot Standard (Catalog # WBC010B)

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Survivin

Survivin is a member of the Inhibitor of Apoptosis (IAP) gene family and contains a single BIR domain. Increased survivin expression at the G2/M checkpoint contributes to tissue homeostasis and differentiation during embryonic and fetal development. Increased expression in differentiated adult tissues, however, frequently results in unchecked cell survival, resulting in cancer. Survivin can protect cells from caspase-induced apoptosis activated in mitosis presumably by binding to Caspase-3, -7, and -9.

Alternate Names

API4, BIRC5

Entrez Gene IDs

332 (Human)

Gene Symbol

BIRC5

Additional Survivin Products

Product Documents for Human Survivin Biotinylated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Survivin Biotinylated Antibody

For research use only

Citations for Human Survivin Biotinylated Antibody

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FAQs

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