Human Thrombomodulin/BDCA-3 Alexa Fluor® 488-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: Thrombomodulin/BDCA-3

Encoded by the THBD gene, Thrombomodulin is also known as CD141 antigen. The deduced amino acid (aa) sequence of human THBD (Accession # P07204) predicts a signal peptide (aa 1-18) and a mature chain (aa 19-575) that consists of following domains: C-type lectin (aa 31-169), EGF-like (aa 241-281, aa 284-324, aa 325-363, aa 365-405, aa 404-440, and aa 441-481), transmembrane (aa 516-539) and cytoplasmic (aa 540-575). The R&D Systems rhTHBD consists of aa 19-515, corresponding to the extracellular portion of the type I membrane protein. Predominantly synthesized by vascular endothelial cells, THBD inhibits coagulation and fibrinolysis (1-3). It functions as a cell surface receptor and an essential cofactor for active thrombin, which in turn activates protein C and thrombin-activatable fibrinolysis inhibitor (TAFI), also known as carboxypeptidase B2 (CPB2). Activated protein C (APC), facilitated by protein S, degrades coagulation factors Va and VIIIa, which are required for thrombin activation. Activated CPB2 cleaves basic C-terminal aa residues of its substrates, including fibrin, preventing the conversion of plasminogen to plasmin. In addition, THBD gene polymorphisims are associated with human disease and THBD plays a role in thrombosis, stroke, arteriosclerosis, and cancer (4). For example, increased serum levels of THBD, due to protease cleavage, have been associated with smoking, cardiac surgery, atherosclerosis, liver cirrhosis, diabetes mellitus, cerebral and myocardial infarction, and multiple sclerosis (5).

Product Datasheets

Related Research Areas

• Coagulation
• Conventional/Classical Dendritic Cells
• Endothelial Cell Coagulation Molecules
• Endothelial Cell Markers
• Monocyte Markers
• Multiple Sclerosis and Myelin Related Molecules
• Neutrophils
• Preeclampsia
• Serine Proteases and Regulators