Human TRA-1-85/CD147 Antibody (TRA-1-85)

R&D Systems | Catalog # MAB3195

R&D Systems
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Rat, Bovine, Parasite - Plasmodium falciparum

Applications

Validated:

Knockout Validated, Western Blot, Flow Cytometry, Immunocytochemistry, CyTOF-ready

Cited:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Neutralization, Immunocytochemistry, Functional Assay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # TRA-1-85
View all TRA-1-85/CD147 Primary Antibodies »

Product Specifications

Immunogen

2120Ep human embryonal carcinoma cell line

Specificity

Detects human TRA‑1‑85 antigen in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human TRA-1-85/CD147 Antibody (TRA-1-85)

Detection of Human TRA-1-85/CD147 antibody by Western Blot.

Detection of Human TRA‑1‑85/CD147 by Western Blot.

Western blot shows lysates of human brain (cerebellum) tissue, human heart tissue, and human liver tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human TRA-1-85/CD147 Monoclonal Antibody (Catalog # MAB3195) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for TRA-1-85/CD147 at approximately 40-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of TRA‑1‑85/CD147 antibody in Human PBMCs antibody by Flow Cytometry.

Detection of TRA‑1‑85/CD147 in Human PBMCs by Flow Cytometry.

Human peripheral blood lymphocytes and monocytes were stained with either (A) Mouse Anti-Human TRA-1-85/CD147 Monoclonal Antibody (Catalog # MAB3195) or (B) Mouse IgG1Isotype Control (Catalog # MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).
TRA-1-85/CD147 antibody in BG01V Human Embryonic Stem Cells by Immunocytochemistry (ICC).

TRA‑1‑85/CD147 in BG01V Human Embryonic Stem Cells.

TRA-1-85/CD147 was detected in immersion fixed BG01V human embryonic stem cells on irradiated mouse embryonic fibroblasts using Mouse Anti-Human TRA-1-85/CD147 Monoclonal Antibody (Catalog # MAB3195) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Western Blot Shows Human TRA-1-85/CD147 Antibody Specificity by Using Knockout Cell Line.

Western Blot Shows Human TRA‑1‑85/CD147 Specificity by Using Knockout Cell Line.

Western blot shows lysates of HEK293T human embryonic kidney parental cell line and TRA-1-85/CD147 knockout HEK293T cell line (KO). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human TRA-1-85/CD147 Monoclonal Antibody (Catalog # MAB3195) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for TRA-1-85/CD147 at approximately 51 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in knockout HEK293T cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of TRA-1-85/CD147 by Western Blot

Detection of TRA-1-85/CD147 by Western Blot

MGMT expression in tumor tissue. (A) Nude mice were injected subcutaneously with 4 × 106 MGMT-positive A375 cells or with MGMT-negative A2058 cells. Once pea-sized tumors had developed, tumor tissue was harvested and subjected to immunostaining for human MGMT. While A375 tissue showed positive staining in most cells, A2058 tissue revealed positivity only in a small minority of cells (2 examples are indicated by arrows in the enlarged section). (B) Western blot was performed with crude lysates from cells cultured in vitro (A375, A2058, M24, and MEF) and from xenograft tumor tissues (A1–A4: tumors derived from implanted A2058 cells; M1–M3: tumors derived from implanted M24 cells), using a human-specific MGMT antibody. A1, A2, M1 and M2 are tumors from mice that only received vehicle treatment (control); A3, A4 and M3 are tumors from mice that received TMZ therapy (M4 animal was lost). To confirm the presence of human tumor cells in tissues harvested from mice—especially in those lanes where the MGMT signal was negative—we used an antibody against TRA-1-85/CD147, an epitope that is present only on human cells, but not mouse cells. Please note that specificity of this antibody was confirmed by lack of reactivity with the lane containing lysate from mouse embryo fibroblasts (MEF). Actin was used as the loading control. Numbers under the blot indicate quantification of MGMT bands, with reference to the actin signal, and relative to A4 sample (set at 1.0); <0.1 indicates below detection limit; only lanes with tumor tissue samples were measured. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/30274152), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human TRA-1-85/CD147 Antibody (TRA-1-85)

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: Human peripheral blood lymphocytes and monocytes

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed BG01V human embryonic stem cells on irradiated mouse embryonic fibroblasts

Knockout Validated

TRA‑1‑85/CD147 is specifically detected in HEK293T human embryonic kidney parental cell line but is not detectable in TRA‑1‑85/CD147 knockout HEK293T cell line.

Western Blot

1 µg/mL
Sample: Human brain (cerebellum) tissue, human heart tissue, and human liver tissue

Reviewed Applications

Read 1 review rated 4 using MAB3195 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TRA-1-85/CD147

The TRA-1-85 antigen, also known as OKa blood group antigen, is a specific epitope within the protein known as Basigin, EMMPRIN and CD147. It is a cell surface determinant expressed on almost all human cell types. This antibody has been used in somatic cell hybrid studies to identify tissues of partial human origin (1, 2).

References

  1. Williams, B.P. et al. (1988) Immunogenetics. 27:322.
  2. Spring, F.A. et al. (1997) Eur. J. Immunol. 27:891.

Alternate Names

OKa Blood Group Antigen, TRA185

Entrez Gene IDs

682 (Human)

Gene Symbol

BSG

Additional TRA-1-85/CD147 Products

Product Documents for Human TRA-1-85/CD147 Antibody (TRA-1-85)

Certificate of Analysis

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Product Specific Notices for Human TRA-1-85/CD147 Antibody (TRA-1-85)

For research use only

Citations for Human TRA-1-85/CD147 Antibody (TRA-1-85)

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  • Human TRA-1-85/CD147 Antibody
    Name: SASAN JALILI
    Application: Immunohistochemistry
    Sample Tested: Cardiac Progenitors
    Species: Human
    Verified Customer | Posted 08/30/2017
    Human TRA-1-85/CD147 Antibody (TRA-1-85) MAB3195

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