Human VDR/NR1I1 Antibody

R&D Systems | Catalog # PP-H4537-00

R&D Systems
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Direct ELISA, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # H4537
View all VDR/NR1I1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant human VDR/NR1I1
aa 91-210

Specificity

This antibody specifically recognizes human VDR and cross reacts with mouse and rat VDR. Not yet tested in other species.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Applications for Human VDR/NR1I1 Antibody

Application
Recommended Usage

Direct ELISA

This antibody can be used at 0.1 μg/mL with the appropriate secondary reagents to detect human VDR.

Immunohistochemistry

This antibody can be used at 20 - 40 μg/mL with the appropriate secondary reagents to detect human VDR.

Immunoprecipitation

Optimal dilutions should be determined by each laboratory.

Western Blot

This antibody can be used at 1 μg/mL under reducing conditions with the appropriate secondary reagents to detect human VDR.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

A liquid formulation in physiologic saline with 0.1% NaN3.

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

This antibody is stable for greater than six months when held at -20 °C in a manual defrost freezer or at -70 °C. Upon thawing, the antibody can be stored at 2-8 °C for at least 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Background: VDR/NR1I1

Vitamin D Receptor (VDR; NR1I1) is a member of the Steroid Receptor family related to the Pregnane X Receptor (PXR) and Constitutive Androstane Receptors (CARs). The natural ligand of VDR is 1, 25 di-hydroxyvitamin D3. VDR is expressed in osteoblasts, osteocytes, osteoclasts, bone, bone marrow, thymus and small intestine. VDR plays critical roles in calcium homeostasis, bone development and mineralization, as well as control of cell growth and differentiation. Retnoid X Receptors (RXRs) are the major partners for VDR since by heterodimerizing with VDR, they increase their DNA-binding affinity and select the correct spacing of direct repeat elements.

Long Name

Vitamin D [1,25-Dihydroxyvitamin D3] Receptor

Alternate Names

NR1I1

Entrez Gene IDs

7421 (Human)

Gene Symbol

VDR

Additional VDR/NR1I1 Products

Product Documents for Human VDR/NR1I1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human VDR/NR1I1 Antibody

For research use only

Citations for Human VDR/NR1I1 Antibody

Customer Reviews for Human VDR/NR1I1 Antibody

There are currently no reviews for this product. Be the first to review Human VDR/NR1I1 Antibody and earn rewards!

Have you used Human VDR/NR1I1 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies