Rabbit IgG Isotype Control [Alexa Fluor® 488]

Novus Biologicals | Catalog # NBP2-24982

Novus Biologicals

Key Product Details

Species Reactivity

Rabbit

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Alexa Fluor 488 (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Polyclonal Rabbit IgG
View all IgG Isotype Controls »

Product Specifications

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This Rabbit IgG immunoglobulin is useful as an isotype control for Rabbit IgG antibody.

Scientific Data Images for Rabbit IgG Isotype Control [Alexa Fluor® 488]

Flow Cytometry: Rabbit IgG Isotype Control [Alexa Fluor® 488] [NBP2-24982]

Flow Cytometry: Rabbit IgG Isotype Control [Alexa Fluor® 488] [NBP2-24982]

Flow Cytometry: Rabbit IgG Isotype Control [Alexa Fluor 488] [NBP2-24982] - Intracellular analysis of TLR1 in human PBMCs (lymphocyte-gated) using this antibody. Shaded histogram represents cells alone, green represents isotype control, red represents TLR1 antibody. TLR intracellular flow kit was used for this test.

Applications for Rabbit IgG Isotype Control [Alexa Fluor® 488]

Application
Recommended Usage

Flow Cytometry

1 ul/1 million cells

Immunocytochemistry/ Immunofluorescence

1:10 - 1:500
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Spectra Viewer

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50 mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please contact technical services for concentration.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: IgG

IgG is a monomeric immunoglobulin, built of two heavy chains gamma and two light chains. Each molecule has two antigen binding sites. This is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglobulins in humans. This is the only isotype that can pass through the human placenta, thereby providing protection to the fetus in its first weeks of life before its own immune system has developed. It can bind to many kinds of pathogens, for example viruses, bacteria, and fungi, and protects the body against them by complement activation (classic pathway), opsonization for phagocytosis and neutralisation of their toxins. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%).

Long Name

Immunoglobulin G

Alternate Names

Immunoglobulin G, ImmunoglobulinG

Gene Symbol

IGHG1

Additional IgG Products

Product Documents for Rabbit IgG Isotype Control [Alexa Fluor® 488]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Rabbit IgG Isotype Control [Alexa Fluor® 488]



Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

Citations for Rabbit IgG Isotype Control [Alexa Fluor® 488]

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  • Rabbit IgG Isotype Control [Alexa Fluor® 488]
    Name: Anonymous
    Verified Customer | Posted 02/01/2018
    ICC staining dog cells.
    This antibody served as a negative control for ICC staining.
    Rabbit IgG Isotype Control [Alexa Fluor® 488] NBP2-24982

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Protocols

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