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Rabbit IgG Isotype Control

Novus Biologicals | Catalog # NBP2-24891

Novus Biologicals

Key Product Details

Species Reactivity

Rabbit

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), In vitro assay

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all IgG Isotype Controls »

Product Specifications

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This Rabbit IgG immunoglobulin is useful as an isotype control for Rabbit IgG antibody.

Scientific Data Images for Rabbit IgG Isotype Control

Immunohistochemistry: Rabbit IgG Isotype Control [NBP2-24891]

Immunohistochemistry: Rabbit IgG Isotype Control [NBP2-24891]

Controls for alpha-MSH immunohistochemistry and immunofluorescence. (A) Isotype controls for alpha-MSH were obtained by staining consecutive sections of human and mouse atherosclerotic plaques with alpha-MSH antibody or rabbit polyclonal isotype control antibody. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/s41598-018-33523-7) licensed under a CC-BY license..
Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891]

Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891]

Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-rabbit secondary antibody. Image using the Azide Free form of this antibody.
Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891]

Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891]

Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.
Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891]

Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891]

Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - PBMCs fixed and permeabilized using the Novus intracellular staining kit and stained with 1 ug of TLR7-PE conjugate (red) or rabbit IgG-PE conjugate isotype control (NBP2-24983, green). Image using the PE form of this antibody.
Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891]

Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891]

Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular analysis of TLR1 in human PBMCs (lymphocyte-gated) using this antibody. Shaded histogram represents cells alone, green represents isotype control, red represents TLR1 antibody. TLR intracellular flow kit was used for this test. Image using the Alexa Fluor 488 form of this antibody.
Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891]

Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891]

Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Analysis of TLR7 in Ramos cells using 2 ug of NBP2-24892. Shaded histogram represents FITC-conjugated rabbit IgG isotype control ; red represents anti-TLR7 antibody. Image using the FITC form of this antibody.
Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891]

Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891]

Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - FLOW analysis of Mouse CD4+ T cells that were stimulated with anti-CD3/CD28 beads and insulin (1ug/mL) for 5 days in culture media with additional glucose provided. PE conjugated Glut1 antibody stained cells (NB110-39113PE, Orange) were included as positive control which were compared with Rb IgG PE, NBP2-24983 as Isotype Control (Blue) as well as with fluorescence minus one/FMO control (Red) [Image submitted by Verified Customer - see Review for Protocol Details]. Image using the PE form of this antibody.
Rabbit IgG Isotype Control

Immunocytochemistry/ Immunofluorescence: Rabbit IgG Isotype Control [NBP2-24891] -

Immunocytochemistry/ Immunofluorescence: Rabbit IgG Isotype Control [NBP2-24891] - The expression & localization of PRCP in the human atherosclerotic plaque. (A) Immunohistochemical staining of PRCP (brown color) in a carotid endarterectomy sample. Scale bars, 500 µm (left) & 50 µm (right). (B) A consecutive section of the carotid sample was immunofluorescently stained for PRCP (red) & Mac-2 (green), & counterstained with DAPI. Cells that clearly express both PRCP & Mac-2 are indicated by white arrows. Scale bars, 500 µm (left) & 50 µm (right). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30305673), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Rabbit IgG Isotype Control

Immunohistochemistry: Rabbit IgG Isotype Control [NBP2-24891] -

Immunohistochemistry: Rabbit IgG Isotype Control [NBP2-24891] - alpha -melanocyte stimulating hormone ( alpha -MSH) is expressed by plaque macrophages in human atheroma. (A) alpha -MSH immunostaining (brown color) of carotid endarterectomy sample. Scale bars, 200 µm (left) & 50 µm (right). (B) A consecutive section of the carotid sample was immunofluorescently stained for alpha -MSH (red) & Mac-2 (green), & counterstained with DAPI. Cells that clearly express both alpha -MSH & Mac-2 are indicated by white arrows. Scale bars, 200 µm (left) & 50 µm (right). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30305673), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Rabbit IgG Isotype Control

Application
Recommended Usage

Chromatin Immunoprecipitation

1:10-1:500. Use reported in scientific literature (PMID 23034403)

Chromatin Immunoprecipitation (ChIP)

1:10-1:500

Flow Cytometry

1:10 - 1:1000

Immunocytochemistry/ Immunofluorescence

reported in scientific literature (PMID 32784470)

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10 - 1:500. Use reported in scientific literature (PMID 255994242)
Application Notes
Use the isotype control at the same concentration as the experimental antibody. Optimal dilution of this antibody should be experimentally determined. In vitro assay reported in multiple scientific literatures with the Azide free version (NBP2-24893)

Reviewed Applications

Read 1 review rated 5 using NBP2-24891 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: IgG

IgG is a monomeric immunoglobulin, built of two heavy chains gamma and two light chains. Each molecule has two antigen binding sites. This is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglobulins in humans. This is the only isotype that can pass through the human placenta, thereby providing protection to the fetus in its first weeks of life before its own immune system has developed. It can bind to many kinds of pathogens, for example viruses, bacteria, and fungi, and protects the body against them by complement activation (classic pathway), opsonization for phagocytosis and neutralisation of their toxins. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%).

Long Name

Immunoglobulin G

Alternate Names

Immunoglobulin G, ImmunoglobulinG

Gene Symbol

IGHG1

Additional IgG Products

Product Documents for Rabbit IgG Isotype Control

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Rabbit IgG Isotype Control

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

Citations for Rabbit IgG Isotype Control

Customer Reviews for Rabbit IgG Isotype Control (1)

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  • Name: Ranveer Singh
    Application: Chromatin Immunoprecipitation
    Sample Tested: Cross-linked chromatin from HEK 293T cells
    Species: Human
    Verified Customer | Posted 06/18/2014
    ChIP on Bcl2 promoter using rabbit IgG as control
    Rabbit IgG Isotype Control NBP2-24891

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Protocols

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