Rabbit IgG Isotype Control

Novus Biologicals | Catalog # NBP2-24893

Novus Biologicals

Key Product Details

Species Reactivity

Rabbit

Applications

Immunohistochemistry, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, In vitro assay

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all IgG Isotype Controls »

Product Specifications

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This Rabbit IgG immunoglobulin is useful as an isotype control for Rabbit IgG antibody.

Scientific Data Images for Rabbit IgG Isotype Control

Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24893]

Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24893]

Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24893] - Intracellular analysis of mouse TLR 6 polyclonal antibody (Red peak), rabbit isotype control (Green peak), RAW cells (Purple peak).Two micrograms of antibodies were used. Goat anti-rabbit FITC was used as secondary antibody.
Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24893]

Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24893]

Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24893] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-rabbit secondary antibody.

Applications for Rabbit IgG Isotype Control

Application
Recommended Usage

Flow Cytometry

1ul/1 million cells

Immunocytochemistry/ Immunofluorescence

1:10 - 1:500. Use reported in scientific literature (PMID 22047818)

Immunohistochemistry

1:10 - 1:500

In vitro assay

reported in scientific literature (PMID 25467444)
Application Notes
Use the isotype control at the same concentration as the experimental antibody. Optimal dilution of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using NBP2-24893 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: IgG

IgG is a monomeric immunoglobulin, built of two heavy chains gamma and two light chains. Each molecule has two antigen binding sites. This is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglobulins in humans. This is the only isotype that can pass through the human placenta, thereby providing protection to the fetus in its first weeks of life before its own immune system has developed. It can bind to many kinds of pathogens, for example viruses, bacteria, and fungi, and protects the body against them by complement activation (classic pathway), opsonization for phagocytosis and neutralisation of their toxins. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%).

Long Name

Immunoglobulin G

Alternate Names

Immunoglobulin G, ImmunoglobulinG

Gene Symbol

IGHG1

Additional IgG Products

Product Documents for Rabbit IgG Isotype Control

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Rabbit IgG Isotype Control

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

Citations for Rabbit IgG Isotype Control

Customer Reviews for Rabbit IgG Isotype Control (1)

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  • Name: Anonymous
    Application: Immunoprecipitation
    Sample Tested: HEK293 cells treated with etoposide (5h)
    Species: Human
    Verified Customer | Posted 08/26/2014
    Immunoprecipitation with rabbit IgG and anti-PARP in 293 cells
    Rabbit IgG Isotype Control NBP2-24893

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Protocols

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FAQs

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