Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry, Flow (Cell Surface), Flow (Intracellular), Immunocytochemistry/ Immunofluorescence

Label

Alexa Fluor 647 (Excitation = 650 nm, Emission = 668 nm)

Antibody Source

Polyclonal Mouse IgG1
View all IgG1 Isotype Controls »

Product Specifications

Clonality

Polyclonal

Host

Mouse

Isotype

IgG1

Description

This Mouse IgG1 immunoglobulin is useful as an isotype control for Mouse IgG1 antibody.

Applications for Mouse IgG1 Isotype Control [Alexa Fluor® 647]

Application
Recommended Usage

Flow (Cell Surface)

1:10 - 1:1000

Flow (Intracellular)

1:10 - 1:1000

Immunocytochemistry/ Immunofluorescence

5 ug/ml

Immunohistochemistry

5 ug/ml
Application Notes
Use the isotype control at the same concentration as the experimental antibody. Optimal dilution of this antibody should be experimentally determined.

Spectra Viewer

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Ion exchange chromatography

Formulation

PBS, 50% glycerol.

Preservative

No Preservative

Concentration

Please contact technical services for concentration.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C in the dark. Avoid freeze-thaw cycles.

Background: IgG1

IgG is a monomeric immunoglobulin composed of two heavy chains and two light chains. There are four subclasses of the IgG: IgG1, IgG2, IgG3 and IgG4. Each molecule has two antigen binding sites. IgG is the most abundant immunoglobulin as well as the only isotype that can pass through the placent,a thereby providing protection to the fetus in its first weeks of life before, its own immune system has developed. IgG can bind to several different kinds of pathogens, for example viruses, bacteria and fungi, and it protects the body against them by complement activation (the classic pathway), opsonization for phagocytosis and neutralization of their toxins.

Long Name

Immunoglobulin G1

Alternate Names

IgG1, Igh-4, RGD1359539, VH7183

Additional IgG1 Products

Product Documents for Mouse IgG1 Isotype Control [Alexa Fluor® 647]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Mouse IgG1 Isotype Control [Alexa Fluor® 647]

This product is manufactured by Eurobio Scientific (formerly Dendritics) and distributed by Novus Biologicals.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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