Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Flow (Intracellular), Immunocytochemistry/ Immunofluorescence, Simple Western, Immunoprecipitation, Gel Super Shift Assays

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence, EMSA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 10A9B6

Format

BSA Free
View all IKK beta Primary Antibodies »

Product Specifications

Immunogen

Full-length human IKKb/IKK2 recombinant protein was used as immunogen (NP_001547).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Novus Biologicals Mouse IKK beta Antibody (10A9B6) - BSA Free (NB100-56513) is a monoclonal antibody validated for use in IHC, WB, Flow, ICC/IF, Simple Western and IP. Anti-IKK beta Antibody: Cited in 9 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for IKK beta Antibody (10A9B6) - BSA Free

Western Blot: IKK beta Antibody (10A9B6)BSA Free [NB100-56513]

Western Blot: IKK beta Antibody (10A9B6)BSA Free [NB100-56513]

Western Blot: IKK beta Antibody (10A9B6) [NB100-56513] - IKK beta in Daudi cell lysate using IKK beta antibody at 5 ug/ml.
Immunohistochemistry-Frozen: IKK beta Antibody (10A9B6) - BSA Free [NB100-56513]

Immunohistochemistry-Frozen: IKK beta Antibody (10A9B6) - BSA Free [NB100-56513]

Immunohistochemistry-Frozen: IKK beta Antibody (10A9B6) [NB100-56513] - Mouse skin tissue section stained with IKK beta antibody. IHC-Fr image submitted by a verified customer review.
Flow (Intracellular): IKK beta Antibody (10A9B6) - BSA Free [NB100-56513]

Flow (Intracellular): IKK beta Antibody (10A9B6) - BSA Free [NB100-56513]

Flow (Intracellular): IKK beta Antibody (10A9B6) [NB100-56513] - Analysis using the Alexa Fluor (R) 488 conjugate. Staining of IKK in HEK 293 cells using 0.1 ug of this antibody. Green histogram represents the isotype control, red represents the IKK antibody. Image using the Azide Free format of this antibody.
Simple Western: IKK beta Antibody (10A9B6)BSA Free [NB100-56513]

Simple Western: IKK beta Antibody (10A9B6)BSA Free [NB100-56513]

Simple Western: IKK beta Antibody (10A9B6) [NB100-56513] - Analysis using Azide Free version of NB100-56513. Lane view shows a specific band for IKK beta in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. *Non-specific interaction with the
Immunoprecipitation: IKK beta Antibody (10A9B6) - BSA Free [NB100-56513]

Immunoprecipitation: IKK beta Antibody (10A9B6) - BSA Free [NB100-56513]

Immunoprecipitation: IKK beta Antibody (10A9B6) [NB100-56513] - Antibody was used to immunoprecipitate IKKb from 1x10^6 Daudi cells and the immuno-precipitated protein was detected by western blotting using NB100-56509. IKKb was detected as a protein of ~87 kD. Image using the Azide Free format of this antibody.

Applications for IKK beta Antibody (10A9B6) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:10 - 1:1000

Gel Super Shift Assays

reported in scientific literature (PMID 19662631)

Immunocytochemistry/ Immunofluorescence

reported in scientific literature (PMID 24825920)

Immunohistochemistry-Frozen

reported by customer review

Immunoprecipitation

2 - 5 ug / 10^6 cells

Simple Western

1:12.5

Western Blot

2 - 5 ug/mL
Application Notes

An approx. 87 kDa band should be observed. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in HeLa lysate 1.0 mg/mL, separated by Size, antibody dilution of 1:12.5, apparent MW was 89 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.

Reviewed Applications

Read 1 review rated 5 using NB100-56513 in the following applications:

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IKK beta

NF-kB (nuclear factor kB) is sequestered in the cytoplasm by IkB family of inhibitory proteins that mask the nuclear localization signal of NF-kB thereby preventing translocation of NF-kB to the nucleus. External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Synthesis of IkBa is autoregulated. IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g. IKK1/a and IKK2/b are phosphorylated by NF-kB-inducing kinase (NIK) and MAP kinase kinase kinase-1 (MEKK1), respectively. Recent studies have shown that IKK2/b, not IKK1/a, is the target proinflammatory stimuli. Targeted disruption of IKK2/b gene in mice also results in extensive liver damage from apotopsis and death as embryo.

Long Name

IkB Kinase beta

Alternate Names

IkBKB, IKK2, NFKBIKB

Entrez Gene IDs

3551 (Human); 16150 (Mouse)

Gene Symbol

IKBKB

UniProt

O14920

Additional IKK beta Products

Product Documents for IKK beta Antibody (10A9B6) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for IKK beta Antibody (10A9B6) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for IKK beta Antibody (10A9B6) - BSA Free

Customer Reviews for IKK beta Antibody (10A9B6) - BSA Free (1)

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Customer Images

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  • IKK beta Antibody (10A9B6)
    Name: Anonymous
    Application: Immunohistochemistry-Frozen
    Sample Tested: mouse skin
    Species: Mouse
    Verified Customer | Posted 08/17/2021
    IKK beta Antibody, mouse skin
    IKK beta Antibody (10A9B6) - BSA Free NB100-56513
    Bio-Techne Response
    This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.

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