IKK beta Antibody (10AG2) - Azide and BSA Free

Name: IKK beta Antibody (10AG2) - Azide and BSA Free
Brand: Novus Biologicals
SKU: NBP2-80788
Price: 419 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-80788

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoblotting, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Simple Western, Immunoprecipitation, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # 10AG2

Format

Azide and BSA Free

View all IKK beta Primary Antibodies »

Product Specifications

Immunogen

A full-length human IKKb recombinant protein was used to immunize mice (NP_001547).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Theoretical MW

86.564 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Mouse IKK beta Antibody (10AG2) - Azide and BSA Free (NB100-56509) is a monoclonal antibody validated for use in IHC, WB, Flow, ICC/IF, Simple Western and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for IKK beta Antibody (10AG2) - Azide and BSA Free

Western Blot: IKK beta Antibody (10AG2)Azide and BSA Free [NBP2-80788]

Western Blot: IKK beta Antibody (10AG2) - Azide and BSA Free [NBP2-80788] - Analysis of A) human Daudi, B) HeLa, and C) mouse NIH3T3 lysate probed with IKKbeta antibody at 2 ug/ml. Image from the standard format of this antibody.

Immunocytochemistry/ Immunofluorescence: IKK beta Antibody (10AG2) - Azide and BSA Free [NBP2-80788]

Immunocytochemistry/Immunofluorescence: IKK beta Antibody (10AG2) - Azide and BSA Free [NBP2-80788] - U-87 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-IKK beta Antibody (10AG2) at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image from the standard format of this antibody.

Immunohistochemistry-Paraffin: IKK beta Antibody (10AG2) - Azide and BSA Free [NBP2-80788]

Immunohistochemistry-Paraffin: IKK beta Antibody (10AG2) - Azide and BSA Free [NBP2-80788] - Human gall bladder probed with IKKbeta antibody at 5 ug/ml. Human tissue TMA was used for this test. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT f

Flow Cytometry: IKK beta Antibody (10AG2) - Azide and BSA Free [NBP2-80788]

Flow Cytometry: IKK beta Antibody (10AG2) - Azide and BSA Free [NBP2-80788] - Intracellular staining of HEK293 cells with 0.5 ug of IKKbeta antibody (red) and 0.5 ug of isotype control antibody (20109, green). Intracellular flow kit was used for this test, and an anti-mouse IgG1 PE conjugated secondary. Image from the standard format of this antibody.

Flow Cytometry: IKK beta Antibody (10AG2) - Azide and BSA Free [NBP2-80788]

Flow Cytometry: IKK beta Antibody (10AG2) - Azide and BSA Free [NBP2-80788] - Analysis of PE conjugate of NB100-56509. An intracellular stain was performed on Jurkat cells with IKK Beta antibody (10AG2) NB100-56509PE (blue) and a matched isotype control NBP2-27287AF488 (orange). Cells were fixed with 4% PFA and then permeablized.

Flow Cytometry: IKK beta Antibody (10AG2) - Azide and BSA Free [NBP2-80788]

Flow Cytometry: IKK beta Antibody (10AG2) - Azide and BSA Free [NBP2-80788] - Analysis using Alexa Fluor (R) 647 conjugate of NB100-56509. An intracellular stain was performed on HeLa cells with IKK beta antibody (10AG2) NB100-56509 (blue) and a matched isotype control NBP2-27287 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. 1 ug of antibody was added to 100 uL of staining buffer and cells were incubated for 30 minutes at room temperature. Both antibodies were directly conjugated to Alexa Fluor 647.

Flow Cytometry: IKK beta Antibody (10AG2) - Azide and BSA Free [NBP2-80788]

Flow Cytometry: IKK beta Antibody (10AG2) - Azide and BSA Free [NBP2-80788] - Analysis using the Alexa Fluor (R) 488 conjugate of NB100-56509. Staining of IKKbeta in HEK 293 cells using 0.1 ug of Alexa Fluor 488-conjugated antibody. Green histogram represents the isotype control, red represents the IKKbeta antibody.

Simple Western: IKK beta Antibody (10AG2)Azide and BSA Free [NBP2-80788]

Simple Western: IKK beta Antibody (10AG2) - Azide and BSA Free [NBP2-80788] - Lane view shows a specific band for IKK beta in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. Image from the standard format of this antibody.

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Applications for IKK beta Antibody (10AG2) - Azide and BSA Free

Application

Recommended Usage

Flow Cytometry

0.25 - 0.5 ug/ 10^6 cells

Immunoblotting

reported in scientific literature (PMID 20103608)

Immunocytochemistry/ Immunofluorescence

2-5 ug/ml. Use reported in scientific literature (PMID 24825920)

Immunohistochemistry

1:10 - 1:500

Immunohistochemistry-Paraffin

reported in scientific literature (Page et al)

Immunoprecipitation

1:10 - 1:500

Simple Western

1:12.5

Western Blot

2 - 4 ug/ml

Application Notes

In Simple Western only 10 - 15 ul of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: antibody dilution of 1:12.5. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. This antibody is CyTOF ready.

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Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: IKK beta

NF-kB (nuclear factor kB) is sequestered in the cytoplasm by IkB family of inhibitory proteins that mask the nuclear localization signal of NF-kB thereby preventing translocation of NF-kB to the nucleus. External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Synthesis of IkBa is autoregulated. IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g. IKK1/a and IKK2/b are phosphorylated by NF-kB-inducing kinase (NIK) and MAP kinase kinase kinase-1 (MEKK1), respectively. Recent studies have shown that IKK2/b, not IKK1/a, is the target proinflammatory stimuli. Targeted disruption of IKK2/b gene in mice also results in extensive liver damage from apotopsis and death as embryo.

Long Name

IkB Kinase beta

Alternate Names

IkBKB, IKK2, NFKBIKB

Gene Symbol

IKBKB

Additional IKK beta Products

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Product Specific Notices for IKK beta Antibody (10AG2) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.