IL-27R alpha/WSX-1/TCCR Antibody (34N4G11) - BSA Free

Novus Biologicals | Catalog # NBP2-19015

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Flow Cytometry, Flow (Cell Surface), CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 34N4G11

Format

BSA Free
View all IL-27 R alpha/WSX-1/TCCR Primary Antibodies »

Product Specifications

Immunogen

A portion of amino acids 200-250 of human IL-27R (WSX-1) was used as the immunogen for this antibody.

Localization

Membrane (with Extracellular and Cytoplasmic domains)

Specificity

IL-27R (WSX-1) is 65-70 kDa protein and is highly expressed in lymphoid tissues such as spleen, lymph nodes and peripheral blood leukocytes including CD4+ and CD8+ T cells, monocytes, DCs and NK cells

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for IL-27R alpha/WSX-1/TCCR Antibody (34N4G11) - BSA Free

Immunohistochemistry-Paraffin: IL-27R alpha/WSX-1/TCCR Antibody (34N4G11) - BSA Free [NBP2-19015]

Immunohistochemistry-Paraffin: IL-27R alpha/WSX-1/TCCR Antibody (34N4G11) - BSA Free [NBP2-19015]

Immunohistochemistry-Paraffin: IL-27 R alpha/WSX-1/TCCR Antibody (34N4G11) [NBP2-19015] - IHC-P detection of IL27RA protein in a section of human renal/kidney cancer tissue using IL27RA antibody (34N4G11) at a concentration of 5ug/ml. The representative image shows membrane-cytoplasmic positivity for IL27RA protein in the cancer cells.
Flow (Cell Surface): IL-27R alpha/WSX-1/TCCR Antibody (34N4G11) - BSA Free [NBP2-19015]

Flow (Cell Surface): IL-27R alpha/WSX-1/TCCR Antibody (34N4G11) - BSA Free [NBP2-19015]

Flow (Cell Surface): IL-27 R alpha/WSX-1/TCCR Antibody (34N4G11) [NBP2-19015] - Analysis using the Alexa Fluor (R) 647 conjugate of NBP2-19015. Surface staining of IL27R on freshly isolated (PPI negative) human PBMCs using IL-27R antibody and CD3 antibody at 10 ul/10^6 cells. Flow kit, mouse IgG2b isotype control antibody, and RBC lysis buffer were used for this test.
Immunohistochemistry-Paraffin: IL-27R alpha/WSX-1/TCCR Antibody (34N4G11) - BSA Free [NBP2-19015]

Immunohistochemistry-Paraffin: IL-27R alpha/WSX-1/TCCR Antibody (34N4G11) - BSA Free [NBP2-19015]

Immunohistochemistry-Paraffin: IL-27 R alpha/WSX-1/TCCR Antibody (34N4G11) [NBP2-19015] - Adenocarcinoma of the stomach probed with IL-27R antibody at 5 ug/ml.
Immunohistochemistry-Paraffin: IL-27R alpha/WSX-1/TCCR Antibody (34N4G11) - BSA Free [NBP2-19015]

Immunohistochemistry-Paraffin: IL-27R alpha/WSX-1/TCCR Antibody (34N4G11) - BSA Free [NBP2-19015]

Immunohistochemistry-Paraffin: IL-27 R alpha/WSX-1/TCCR Antibody (34N4G11) [NBP2-19015] - FFPE human prostate probed with IL-27R antibody at 5 ug/ml.
Immunohistochemistry-Paraffin: IL-27R alpha/WSX-1/TCCR Antibody (34N4G11) - BSA Free [NBP2-19015]

Immunohistochemistry-Paraffin: IL-27R alpha/WSX-1/TCCR Antibody (34N4G11) - BSA Free [NBP2-19015]

Immunohistochemistry-Paraffin: IL-27 R alpha/WSX-1/TCCR Antibody (34N4G11) [NBP2-19015] - IHC-P detection of IL27RA protein in a section of normal human colon tissue using IL27RA antibody (34N4G11) at a concentration of 5ug/ml. Intense membrane with cytoplasmic staining was observed in the cells of crypts and the absorptive columnar epithelium.
Immunohistochemistry-Paraffin: IL-27R alpha/WSX-1/TCCR Antibody (34N4G11) - BSA Free [NBP2-19015]

Immunohistochemistry-Paraffin: IL-27R alpha/WSX-1/TCCR Antibody (34N4G11) - BSA Free [NBP2-19015]

Immunohistochemistry-Paraffin: IL-27 R alpha/WSX-1/TCCR Antibody (34N4G11) [NBP2-19015] - IHC-P detection of IL27RA protein in a section of human colo-rectal cancer tissue using IL27RA antibody (34N4G11) at a concentration of 5ug/ml. The cancer cells showed specific positivity for IL27RA and the staining intensity was more towards the cellular membranes. The tumor stroma was largely negative for staining.
Immunohistochemistry-Paraffin: IL-27R alpha/WSX-1/TCCR Antibody (34N4G11) - BSA Free [NBP2-19015]

Immunohistochemistry-Paraffin: IL-27R alpha/WSX-1/TCCR Antibody (34N4G11) - BSA Free [NBP2-19015]

Immunohistochemistry-Paraffin: IL-27 R alpha/WSX-1/TCCR Antibody (34N4G11) [NBP2-19015] - IHC-P detection of IL27RA protein in a section of human stomach cancer tissue using IL27RA antibody (34N4G11) at a concentration of 5ug/ml. The cancer cells showed intense membrane-cytoplasmic positivity for IL27RA protein, whereas, the glands in adjacent area and the associated stroma developed distinctly weak staining.
Flow Cytometry: IL-27R alpha/WSX-1/TCCR Antibody (34N4G11) - BSA Free [NBP2-19015]

Flow Cytometry: IL-27R alpha/WSX-1/TCCR Antibody (34N4G11) - BSA Free [NBP2-19015]

Flow Cytometry: IL-27 R alpha/WSX-1/TCCR Antibody (34N4G11) [NBP2-19015] - Surface staining on human peripheral blood monocytes using IL-27R antibody at 1 ug/10^6 cells (red represents specific antibody and green represents isotype control). APC-conjugated goat anti-mouse Ig F(ab')2 secondary antibody, CS flow kit and an FcR block were used for this test.

Applications for IL-27R alpha/WSX-1/TCCR Antibody (34N4G11) - BSA Free

Application
Recommended Usage

Flow (Cell Surface)

1 ug/ml

Flow Cytometry

1 ug/ml

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

5 ug/ml
Application Notes
This antibody is Cytof ready.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IL-27 R alpha/WSX-1/TCCR

The IL-27 Receptor complex includes WSX-1 and gp130. IL-27R (WSX-1) is 65-70 kDa protein and is highly expressed in lymphoid tissues such as spleen, lymph nodes and peripheral blood leukocytes including CD4+ and CD8+ T cells, monocytes, DCs and NK cells (1-3). IL-27R activation includes STAT1, STAT3, STAT4 and STAT5, leading to differing pathway dependent effector activities. Like the IL12/IL-12R complex, the IL-27/IL-27R complex also has Th1 enhancing activity especially enhancement of IFNg production by naive T cells and NK cells (1). IL-27R mediates downregulation of Th2 activity by findings that IL-27R deficiencies mitigate inflammatory pathology in helminthic infections in mice (1, 4, 5). The possibility of utilizing IL-27R as a target for autoimmune inflammatory diseases, especially of mucosal surfaces as in human IBD, is under investigation, measured by Th2 type cytokines and bolstered through Th2

Long Name

Interleukin-27 Receptor Subunit alpha

Alternate Names

IL-27 R alpha, IL-27Ra, IL27R alpha, IL27RA, TCCR, WSX-1

Entrez Gene IDs

9466 (Human)

Gene Symbol

IL27RA

OMIM

605350 (Human)

UniProt

Q6UWB1

Additional IL-27 R alpha/WSX-1/TCCR Products

Product Documents for IL-27R alpha/WSX-1/TCCR Antibody (34N4G11) - BSA Free

Certificate of Analysis

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Product Specific Notices for IL-27R alpha/WSX-1/TCCR Antibody (34N4G11) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for IL-27R alpha/WSX-1/TCCR Antibody (34N4G11) - BSA Free

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Protocols

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FAQs

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Associated Pathways

Th1 Differentiation Pathway
Th1 Differentiation Pathway Thumbnail