Integrin beta 2/CD18 Antibody (YFC118.3) - BSA Free

Novus Biologicals | Catalog # NB200-610

Clone YFC118.3 was used by HLDA to establish CD designation.
Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Frozen, Flow Cytometry, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # YFC118.3

Format

BSA Free
View all Integrin beta 2/CD18 Primary Antibodies »

Product Specifications

Immunogen

Human neutrophils

Reactivity Notes

Predicted cross-reactivities: Dog, Guinea Pig

Specificity

This recognises the human CD18 cell surface antigen, the integrin beta2 subunit. CD18 is expressed by the majority of leucocytes.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Integrin beta 2/CD18 Antibody (YFC118.3) - BSA Free

Immunocytochemistry/ Immunofluorescence: Integrin beta 2/CD18 Antibody (YFC118.3) - BSA Free [NB200-610]

Immunocytochemistry/ Immunofluorescence: Integrin beta 2/CD18 Antibody (YFC118.3) - BSA Free [NB200-610]

Immunocytochemistry/Immunofluorescence: Integrin beta 2/CD18 Antibody (YFC118.3) [NB200-610] - A-D Confocal microscopy of IBA-1 (green staining) immunohistochemistry of RPE flatmounts (RPE autofluorescence visible as orange due to its autofluorescence in the red and green channel) from a healthy donor (A), a geographic atrophy lesion (B), and large drusen (C and D). (A, B, D): orthogonal Z-stack projection; (C): oblique Z-stack projection and dissecting microscope appearance of postmortem large drusen after removal of the overlaying retina (inset). E-G Double-labeling on the subretinal side of the retina (to avoid masking by RPE autofluorescence) of IBA-1+ (E, green fluorescence) and CD18 (F, red fluorescence; G, merge). H. Orthogonal and lateral Z-stack of a subretinal IBA-1+ (green fluorescence) MPs adjacent to the RPE (orange autofluorescence) in the vicinity of a large drusen.
Immunohistochemistry-Frozen: Integrin beta 2/CD18 Antibody (YFC118.3) - BSA Free [NB200-610]

Immunohistochemistry-Frozen: Integrin beta 2/CD18 Antibody (YFC118.3) - BSA Free [NB200-610]

Immunohistochemistry-Frozen: Integrin beta 2/CD18 Antibody (YFC118.3) [NB200-610] - Canine histiocytic sarcoma stained with Integrin beta 2/CD18 Antibody (YFC118.3). IHC-Fr image submitted by a verified customer review.
Flow Cytometry: Integrin beta 2/CD18 Antibody (YFC118.3) - BSA Free [NB200-610]

Flow Cytometry: Integrin beta 2/CD18 Antibody (YFC118.3) - BSA Free [NB200-610]

Flow Cytometry: Integrin beta 2/CD18 Antibody (YFC118.3) [NB200-610] - Staining of human peripheral blood granulocytes with RAT ANTI HUMAN CD18: FITC.
Immunocytochemistry/ Immunofluorescence: Integrin beta 2/CD18 Antibody (YFC118.3) - BSA Free [NB200-610]

Immunocytochemistry/ Immunofluorescence: Integrin beta 2/CD18 Antibody (YFC118.3) - BSA Free [NB200-610]

Immunocytochemistry/Immunofluorescence: Integrin beta 2/CD18 Antibody (YFC118.3) [NB200-610] - Analysis of canine thoracic fluid samples using anti-Integrin beta 2 antibody. The antibody showing positive cell surface staining. ICC/IF image submitted by a verified customer review.
Flow Cytometry: Integrin beta 2/CD18 Antibody (YFC118.3) - BSA Free [NB200-610]

Flow Cytometry: Integrin beta 2/CD18 Antibody (YFC118.3) - BSA Free [NB200-610]

Flow Cytometry: Integrin beta 2/CD18 Antibody (YFC118.3) [NB200-610] - Staining of human peripheral blood lymphocytes with Rat anti Human CD18.
Flow Cytometry: Integrin beta 2/CD18 Antibody (YFC118.3) - BSA Free [NB200-610]

Flow Cytometry: Integrin beta 2/CD18 Antibody (YFC118.3) - BSA Free [NB200-610]

Flow Cytometry: Integrin beta 2/CD18 Antibody (YFC118.3) [NB200-610] - A) Isotype control with macrophages showing delineation of the M1 marker based on the negative population. B and C represent expression of CD18 (B) or CD11b (C) before incubation with Leishmania. D and E represent expression of CD18 (D) or CD11b (E) after association with Leishmania in a SD assay. In all cases the percent positive cells within the M1 marker are identified. This Figure is a representative diagram of 4 experiments with similar findings.
Flow Cytometry: Integrin beta 2/CD18 Antibody (YFC118.3) - BSA Free [NB200-610]

Flow Cytometry: Integrin beta 2/CD18 Antibody (YFC118.3) - BSA Free [NB200-610]

Flow Cytometry: Integrin beta 2/CD18 Antibody (YFC118.3) [NB200-610] - LFA-1 activation analyses by Flow Cytometry. (A) Profiles of CD11a, CD18 activation epitope (CD18act, representing high affinity conformation) and CD18 (CD18tot) expression by Teff and Treg#1 cells pre-incubated or not with indicated antibodies. Anti-CD28 and anti-CTLA-4 were used at 10 ug/ml. (B) Histograms of Mean Fluorescent Intensity (MFI) of CD11a, CD18act and CD18tot expressed on Teff and Treg#1 cells. Ratio CD18act MFI: CD18tot was established to analyze LFA-1 high affinity conformation in indicated conditions. Data are representative of more than three different experiments. Filled gray, negative control; Red line, Teff and Treg cells alone; Green line, cells with APC; Blue line, cells with APC and anti-CD28; and Black line: cells with APC, anti-CD28 and anti-CTLA-4.

Applications for Integrin beta 2/CD18 Antibody (YFC118.3) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:50-1:100

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:1000-1:5000

Immunoprecipitation

1:10-1:500

Reviewed Applications

Read 2 reviews rated 4.5 using NB200-610 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Integrin beta 2/CD18

CD18 is an integrin. Integrins are integral cell surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. For example, beta 2 combines with the alpha L chain to form the integrin LFA1, and combines with the alpha M chain to form the integrin Mac1. Integrins are known to participate in cell adhesion as well as cell surface mediated signalling. CD18 is expressed by most leucocytes. Defects in the CD18 gene result in leukocyte adhesion deficiency.

Alternate Names

CD18, ITGB2, LCAMB, LFA-1, LFA-1 beta, MAC-1 beta, MF17, p150,95 beta

Gene Symbol

ITGB2

UniProt

P05107 (Human)

Additional Integrin beta 2/CD18 Products

Product Documents for Integrin beta 2/CD18 Antibody (YFC118.3) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Integrin beta 2/CD18 Antibody (YFC118.3) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Integrin beta 2/CD18 Antibody (YFC118.3) - BSA Free (2)

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Customer Images

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  • Integrin beta 2/CD18 Antibody (YFC118.3)
    Name: Anonymous
    Application: Immunohistochemistry-Frozen
    Sample Tested: histiocytic sarcoma cell line and histiocytic sarcoma
    Species: Canine
    Verified Customer | Posted 09/04/2021
    Integrin beta 2/CD18 Antibody in canine histiocytic sarcoma
    Integrin beta 2/CD18 Antibody (YFC118.3) - BSA Free NB200-610
  • Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested:
    Species: Other
    Verified Customer | Posted 01/23/2015
    Integrin beta 2/CD18 Antibody (YFC118.3) - BSA Free NB200-610

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Protocols

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FAQs for Integrin beta 2/CD18 Antibody (YFC118.3) - BSA Free

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  • Q: Can you please provide me the immunogen sequence?

    A: NB200-610 is a licensed clone and all of our available information comes from the clone inventor. Unfortunately, there is no additional information on the immunogen available.

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