JunD Antibody (23GB6560)

Name: JunD Antibody (23GB6560)
Brand: Novus Biologicals
SKU: NBP3-51401
Price: 499 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-51401

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Product Documents

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 23GB6560

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Product Specifications

Immunogen

A synthesized peptide derived from human JunD (Uniprot: P17535)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for JunD Antibody (23GB6560)

Immunocytochemistry/ Immunofluorescence: JunD Antibody (23GB6560)[NBP3-51401] -

JunD antibody (1:1,000), Top panel: wild-type (WT); Bottom panal: JunD shRNA knockdown (KD). Nuclei were stained blue with DAPI; JunD was stained magenta with Alexa Fluor(R) 647. Scale bar, 20 um. Permeabilization: Triton.

Immunocytochemistry/ Immunofluorescence: JunD Antibody (23GB6560)[NBP3-51401] -

Immunocytochemical staining of HepG2 cells with JunD antibody (1:1,000). Nuclei were stained blue with DAPI; JunD was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.

Flow Cytometry: JunD Antibody (23GB6560)[NBP3-51401] -

Validation of JunD knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with JunD antibody (1:2,000) and analyzed using BD flow cytometer.

Flow Cytometry: JunD Antibody (23GB6560)[NBP3-51401] -

Flow cytometric analysis of JunDexpression in HepG2 cells using JunD antibody (1:2,000). Green, isotype control; red, JunD.

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Applications for JunD Antibody (23GB6560)

Application

Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry Panel Builder

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Advanced Features

Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
Spillover Popups - Visualize the spectra of individual fluorochromes
Antigen Density Selector - Match fluorochrome brightness with antigen density

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Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: JunD

The activator protein-1 (AP-1) transcription factor consists of either Jun/Jun homodimers or Fos/Jun heterodimeric complexes. Homo- and heterodimers bind to the TGACTCA consensus sequence present in numerous promoters and initially identified as the phorbol ester tumor promoter response element (TRE). Jun B and Jun D have been shown to be almost identical to c-Jun in their C-terminal regions, which are involved in dimerization and DNA binding, whereas their N-terminal domains, which are involved in transcriptional activation, diverge. All three form heterodimers among themselves and with c-Fos and other members of the Fos gene family. Studies suggest that the two forms of Jun D may be due to internal initiation of translation.

Long Name

JunD Proto-oncogene

Alternate Names

AP-1

Gene Symbol

JUND

Additional JunD Products

Product Documents for JunD Antibody (23GB6560)

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Certificate of Analysis

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Product Specific Notices for JunD Antibody (23GB6560)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.