LDLR Antibody (SJ0197)

Flow Cytometry: LDLR Antibody (SJ0197) [NBP2-67414]

Flow Cytometry: LDLR Antibody (SJ0197) [NBP2-67414] - Analysis of Hela cells with LDL Receptor antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Immunocytochemistry/ Immunofluorescence: LDLR Antibody (SJ0197) [NBP2-67414]

Immunocytochemistry/Immunofluorescence: LDLR Antibody (SJ0197) [NBP2-67414] - Staining LDL Receptor in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/ Immunofluorescence: LDLR Antibody (SJ0197) [NBP2-67414]

Immunocytochemistry/Immunofluorescence: LDLR Antibody (SJ0197) [NBP2-67414] - Staining LDL Receptor in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: LDLR Antibody (SJ0197) [NBP2-67414]

Immunohistochemistry-Paraffin: LDLR Antibody (SJ0197) [NBP2-67414] - Analysis of paraffin-embedded human liver cancer tissue using anti-LDL Receptor antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: LDLR Antibody (SJ0197) [NBP2-67414]

Immunohistochemistry-Paraffin: LDLR Antibody (SJ0197) [NBP2-67414] - Analysis of paraffin-embedded human liver tissue using anti-LDL Receptor antibody. Counter stained with hematoxylin.

Western Blot: LDLR Antibody (SJ0197) [NBP2-67414]

Western blot analysis on different lysates with NBP2-67414 at 1/5,000 dilution. Lane 1: HEK-293 cell lysate (low expression) (20 µg/Lane) Lane 2: Huh7 cell lysate (20 µg/Lane) Lane 3: NIH/3T3 cell lysate (20 µg/Lane) Lane 4: Mouse liver tissue lysate (30 µg/Lane) Lane 5: Rat liver tissue lysate (30 µg/Lane) Predicted band size: 95 kDa Observed band size: 150 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP2-67414 at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Western Blot: LDLR Antibody (SJ0197) [NBP2-67414]

Western blot analysis of PC-12 cell lysates with NBP2-67414 at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 95 kDa Observed band size: 140 kDa Exposure time: 6 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP2-67414 at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.