Lipocalin-2/NGAL Antibody (28) - BSA Free
Novus Biologicals | Catalog # NBP1-30006
![Immunohistochemistry: Lipocalin-2/NGAL Antibody (28) - BSA Free [NBP1-30006]](https://resources.rndsystems.com/images/products/Lipocalin-2-NGAL-Antibody-28-BSA-Free-Immunohistochemistry-NBP1-30006-img0002.jpg "Immunohistochemistry: Lipocalin-2/NGAL Antibody (28) - BSA Free [NBP1-30006]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Porcine
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 28
Format
BSA Free
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Product Specifications
Immunogen
Recombinant pig NGAL
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for Lipocalin-2/NGAL Antibody (28) - BSA Free
Immunohistochemistry: Lipocalin-2/NGAL Antibody (28) - BSA Free [NBP1-30006]
Immunohistochemistry: Lipocalin-2/NGAL Antibody (28) - BSA Free [NBP1-30006] - NGAL expression in the ischemic injured pig kidney; detected with anti-pig NGAL antibody (antibody dilution 1:25).![Immunohistochemistry-Paraffin: Lipocalin-2/NGAL Antibody (28) - BSA Free [NBP1-30006]](https://resources.rndsystems.com/images/products/Lipocalin-2-NGAL-Antibody-28-BSA-Free-Immunohistochemistry-Paraffin-NBP1-30006-img0001.jpg "Immunohistochemistry-Paraffin: Lipocalin-2/NGAL Antibody (28) - BSA Free [NBP1-30006]")
Immunohistochemistry-Paraffin: Lipocalin-2/NGAL Antibody (28) - BSA Free [NBP1-30006]
Immunohistochemistry-Paraffin: Lipocalin-2/NGAL Antibody (28) - BSA Free [NBP1-30006] - Positive staining of renal tubular cell cytoplasm, using mouse monoclonal antibody against pig NGAL. Courtesy of Oscar McCook, University of Ulm.![ELISA: Lipocalin-2/NGAL Antibody (28) - BSA Free [NBP1-30006]](https://resources.rndsystems.com/images/products/Lipocalin-2-NGAL-Antibody-28-BSA-Free-ELISA-NBP1-30006-img0003.jpg "ELISA: Lipocalin-2/NGAL Antibody (28) - BSA Free [NBP1-30006]")
ELISA: Lipocalin-2/NGAL Antibody (28) - BSA Free [NBP1-30006]
ELISA: Lipocalin-2/NGAL Antibody (28) - BSA Free [NBP1-30006] - The figure illustrates the calibration curve of a sandwich assay with NBP1-30005 as the capture antibody and NBP1-30006B as the biotinylated detection antibody.Applications for Lipocalin-2/NGAL Antibody (28) - BSA Free
Application
Recommended Usage
ELISA
1:40000
Western Blot
1:100-1:2000
Application Notes
ELISA: NBP1-30006 binds free pig NGAL in solution. NBP1-30006 (as biotinylated detection antibody) forms a sandwich ELISA pair with NBP1-30005 (as capture antibody) for measuring porcine NGAL.
WB: NBP1-30006 can be used in Western blotting.
IHC: NBP1-30006 detects NGAL in paraffin-embedded sections in the cytoplasm of renal tubular cell from pig.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: Lipocalin-2/NGAL
Long Name
Neutrophil Gelatinase-associated Lipocalin
Alternate Names
24p3, LCN2, Lipocalin2, MSFI, NGAL, Oncogene 24p3, p25, Siderocalin, Uterocalin
Gene Symbol
LCN2
Additional Lipocalin-2/NGAL Products
Product Documents for Lipocalin-2/NGAL Antibody (28) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Lipocalin-2/NGAL Antibody (28) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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