LXR alpha/NR1H3 Antibody (JA20-38)

Novus Biologicals | Catalog # NBP2-66938

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JA20-38 expressed in HEK293
View all LXR alpha/NR1H3 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide within Human LXR alpha/NR1H3 aa 49-98 / 447. (SwissProt: Q13133 Human; SwissProt: Q9Z0Y9 Mouse; SwissProt: Q62685 Rat)

Localization

Nucleus, Cytoplasm.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for LXR alpha/NR1H3 Antibody (JA20-38)

Western Blot: LXR alpha/NR1H3 Antibody (JA20-38) [NBP2-66938]

Western Blot: LXR alpha/NR1H3 Antibody (JA20-38) [NBP2-66938]

Western Blot: LXR alpha/NR1H3 Antibody (JA20-38) [NBP2-66938] - Analysis of LXR alpha/NR1H3 on rat brain tissue lysates with Rabbit anti-LXR alpha antibody at 1/500 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 50 kDa Observed band size: 50 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry/ Immunofluorescence: LXR alpha/NR1H3 Antibody (JA20-38) [NBP2-66938]

Immunocytochemistry/ Immunofluorescence: LXR alpha/NR1H3 Antibody (JA20-38) [NBP2-66938]

Immunocytochemistry/Immunofluorescence: LXR alpha/NR1H3 Antibody (JA20-38) [NBP2-66938] - Staining LXR alpha/NR1H3 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: LXR alpha/NR1H3 Antibody (JA20-38) [NBP2-66938]

Immunohistochemistry-Paraffin: LXR alpha/NR1H3 Antibody (JA20-38) [NBP2-66938]

Immunohistochemistry-Paraffin: LXR alpha/NR1H3 Antibody (JA20-38) [NBP2-66938] - Analysis of paraffin-embedded mouse testes tissue using anti-LXR alpha/NR1H3 antibody. Counter stained with hematoxylin.
Flow Cytometry: LXR alpha/NR1H3 Antibody (JA20-38) [NBP2-66938]

Flow Cytometry: LXR alpha/NR1H3 Antibody (JA20-38) [NBP2-66938]

Flow Cytometry: LXR alpha/NR1H3 Antibody (JA20-38) [NBP2-66938] - Analysis of HepG2 cells with LXR alpha/NR1H3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Western Blot: LXR alpha/NR1H3 Antibody (JA20-38) [NBP2-66938]

Western Blot: LXR alpha/NR1H3 Antibody (JA20-38) [NBP2-66938]

Western Blot: LXR alpha/NR1H3 Antibody (JA20-38) [NBP2-66938] - Analysis of LXR alpha on mouse colon tissue lysate using anti-LXR alpha/NR1H3 antibody at 1/1,000 dilution.
Western Blot: LXR alpha/NR1H3 Antibody (JA20-38) [NBP2-66938]

Western Blot: LXR alpha/NR1H3 Antibody (JA20-38) [NBP2-66938]

Western Blot: LXR alpha/NR1H3 Antibody (JA20-38) [NBP2-66938] - Analysis of LXR alpha/NR1H3 on human liver tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Western Blot: LXR alpha/NR1H3 Antibody (JA20-38) [NBP2-66938]

Western Blot: LXR alpha/NR1H3 Antibody (JA20-38) [NBP2-66938]

Western Blot: LXR alpha/NR1H3 Antibody (JA20-38) [NBP2-66938] - Analysis of LXR alpha/NR1H3 on mouse colon tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.
Immunohistochemistry-Paraffin: LXR alpha/NR1H3 Antibody (JA20-38) [NBP2-66938]

Immunohistochemistry-Paraffin: LXR alpha/NR1H3 Antibody (JA20-38) [NBP2-66938]

Immunohistochemistry-Paraffin: LXR alpha/NR1H3 Antibody (JA20-38) [NBP2-66938] - Analysis of paraffin-embedded human placenta tissue using anti-LXR alpha/NR1H3 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: LXR alpha/NR1H3 Antibody (JA20-38) [NBP2-66938]

Immunohistochemistry-Paraffin: LXR alpha/NR1H3 Antibody (JA20-38) [NBP2-66938]

Immunohistochemistry-Paraffin: LXR alpha/NR1H3 Antibody (JA20-38) [NBP2-66938] - Analysis of paraffin-embedded human spleen tissue using anti-LXR alpha/NR1H3 antibody. Counter stained with hematoxylin.
LXR alpha/NR1H3 Antibody (JA20-38) Western Blot: LXR alpha/NR1H3 Antibody (JA20-38) [NBP2-66938]

Western Blot: LXR alpha/NR1H3 Antibody (JA20-38) [NBP2-66938]

Western blot analysis on different lysates with NBP2-66938 at 1/5,000 dilution. Lane 1: Jurkat cell lysate (15 µg/Lane) Lane 2: HeLa cell lysate (15 µg/Lane) Lane 3: Mouse stomach tissue lysate (20 µg/Lane) Lane 4: Mouse lung tissue lysate (20 µg/Lane) Lane 5: Rat liver tissue lysate (20 µg/Lane) Lane 6: Rat lung tissue lysate (20 µg/Lane) Predicted band size: 50 kDa Observed band size: 50 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP2-66938 at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
LXR alpha/NR1H3 Antibody (JA20-38) Western Blot: LXR alpha/NR1H3 Antibody (JA20-38) [NBP2-66938]

Western Blot: LXR alpha/NR1H3 Antibody (JA20-38) [NBP2-66938]

Western blot analysis on different lysates with NBP2-66938 at 1/5,000 dilution. Lane 1: Jurkat cell lysate (20 µg/Lane) Lane 2: HepG2 cell lysate (20 µg/Lane) Lane 3: Huh7 cell lysate (20 µg/Lane) Lane 4: Mouse lung tissue lysate (40 µg/Lane) Lane 5: Rat liver tissue lysate (40 µg/Lane) Lane 6: Rat lung tissue lysate (40 µg/Lane) Predicted band size: 50 kDa Observed band size: 50 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP2-66938 at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Applications for LXR alpha/NR1H3 Antibody (JA20-38)

Application
Recommended Usage

Flow Cytometry

1:50-1:100

Immunocytochemistry/ Immunofluorescence

1:50-1:100

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:500-1:2000

Reviewed Applications

Read 1 review rated 5 using NBP2-66938 in the following applications:

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: LXR alpha/NR1H3

Liver X Receptors (LXRs) are oxysterol activated nuclear receptors which are involved in the regulation of cholesterol metabolism and bile acids. Two known isoforms, alpha and beta, are differentially expressed. The alpha isoform is predominantly expressed in liver, whereas the beta isoform expression has been shown to be ubiquitous.

LXR antibodies are useful for lipid and cholesterol metabolism studies.

Long Name

Liver X Receptor alpha

Alternate Names

NR1H3, RLD-1

Gene Symbol

NR1H3

Additional LXR alpha/NR1H3 Products

Product Documents for LXR alpha/NR1H3 Antibody (JA20-38)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for LXR alpha/NR1H3 Antibody (JA20-38)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for LXR alpha/NR1H3 Antibody (JA20-38) (1)

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  • LXR Antibody (JA20-38)
    Name: Gaurav Sarode
    Application: Western Blot
    Sample Tested: mouse liver
    Species: Mouse
    Verified Customer | Posted 03/21/2019
    Mouse liver samples: Antibodies give 2 bands, which might be LXR alpha and beta
    LXR alpha/NR1H3 Antibody (JA20-38) NBP2-66938

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