Ly-6B.2 Antibody (7/4) - BSA Free
Novus Biologicals | Catalog # NBP2-13077
![Immunocytochemistry/ Immunofluorescence: Ly-6B.2 Antibody (7/4) - BSA Free [NBP2-13077]](https://resources.rndsystems.com/images/products/Ly-6B-2-Antibody-7-4-Immunocytochemistry-Immunofluorescence-NBP2-13077-img0006.jpg "Immunocytochemistry/ Immunofluorescence: Ly-6B.2 Antibody (7/4) - BSA Free [NBP2-13077]")
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Cited:
Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, IF/IHC
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 7/4
Format
BSA Free
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Product Specifications
Immunogen
Cultured bone marrow cells
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Rat (84%).
Specificity
Recognizes Ly6B.2, a polymorphic 40 kDa antigen expressed by polymorphonuclear cells, but absent on resident tissue macrophages. Strains positive for 7/4:129J; AKR; C57BL/6; C57BL/10; C58; DBA/2; NZB; NZW; SJL; MFI; Swiss (PO) Strains Negative/Weak for 7/4:A2G; A/Sn; ASW; BALB/c; C3H/HEH: CBA.T6T6.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Description
Fusion Partners Spleen cells from AO rats were fused with cells from the Y3 Ag1.2.3 rat myeloma cell line.
Scientific Data Images for Ly-6B.2 Antibody (7/4) - BSA Free
Immunocytochemistry/ Immunofluorescence: Ly-6B.2 Antibody (7/4) - BSA Free [NBP2-13077]
Immunocytochemistry/Immunofluorescence: Ly-6B.2 Antibody (7/4) [NBP2-13077] - IF staining of mouse Peyer's patch with Ly-6B.2 Antibody (7/4), NBP2-13077, green; counterstained for macrophages (F4/80), red and nuclei, blue.![Immunohistochemistry-Paraffin: Ly-6B.2 Antibody (7/4) - BSA Free [NBP2-13077]](https://resources.rndsystems.com/images/products/Ly-6B-2-Antibody-7-4-Immunohistochemistry-Paraffin-NBP2-13077-img0007.jpg "Immunohistochemistry-Paraffin: Ly-6B.2 Antibody (7/4) - BSA Free [NBP2-13077]")
Immunohistochemistry-Paraffin: Ly-6B.2 Antibody (7/4) - BSA Free [NBP2-13077]
Immunohistochemistry-Paraffin: Ly-6B.2 Antibody (7/4) [NBP2-13077] - Ly-6B.2 immunoreactivity in mouse bone marrow. Primary antibody was used at 125 ng/mL and was incubated with tissue sections for 30 minutes at room temperature. IHC-P image submitted by a verified customer review.![Immunohistochemistry-Frozen: Ly-6B.2 Antibody (7/4) - BSA Free [NBP2-13077]](https://resources.rndsystems.com/images/products/Ly-6B-2-Antibody-7-4-Immunohistochemistry-Frozen-NBP2-13077-img0005.jpg "Immunohistochemistry-Frozen: Ly-6B.2 Antibody (7/4) - BSA Free [NBP2-13077]")
Immunohistochemistry-Frozen: Ly-6B.2 Antibody (7/4) - BSA Free [NBP2-13077]
Immunohistochemistry-Frozen: Ly-6B.2 Antibody (7/4) [NBP2-13077] - Staining of C57BL6 mouse spleen cryosection with Ly-6B.2 antibody (7/4).Applications for Ly-6B.2 Antibody (7/4) - BSA Free
Application
Recommended Usage
Flow Cytometry
1:50 - 1:100
Immunocytochemistry/ Immunofluorescence
1:10 - 1:500
Immunohistochemistry
1:10 - 1:500
Immunohistochemistry-Frozen
1:10 - 1:500
Immunohistochemistry-Paraffin
1:10 - 1:500
Western Blot
1:100 - 1:2000
Reviewed Applications
Read 1 review rated 5 using NBP2-13077 in the following applications:
Flow Cytometry Panel Builder
Bio-Techne Knows Flow Cytometry
Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.
Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Product Documents for Ly-6B.2 Antibody (7/4) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Ly-6B.2 Antibody (7/4) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for Ly-6B.2 Antibody (7/4) - BSA Free
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Application: Immunohistochemistry-ParaffinSample Tested: bone marrowSpecies: MouseVerified Customer | Posted 11/01/2021Ly-6B.2 immunoreactivity in mouse bone marrow. Primary was used at 125ng/mL and was incubated for 30m at room temperature.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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