M-CSFR/CD115 Antibody (AFS98) - BSA Free

Novus Biologicals | Catalog # NBP1-43363

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Block/Neutralize, Flow Cytometry

Cited:

Block/Neutralize

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2a Kappa Clone # AFS98

Format

BSA Free
View all M-CSF R/CD115 Primary Antibodies »

Product Specifications

Immunogen

The immunogen for this antibody was CD115.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2a Kappa

Scientific Data Images for M-CSFR/CD115 Antibody (AFS98) - BSA Free

Immunohistochemistry-Paraffin: M-CSFR/CD115 Antibody (AFS98) - BSA Free [NBP1-43363]

Immunohistochemistry-Paraffin: M-CSFR/CD115 Antibody (AFS98) - BSA Free [NBP1-43363]

Immunohistochemistry-Paraffin: M-CSFR/CD115 Antibody (AFS98) [NBP1-43363] - Mouse tumor tissue stained with M-CSFR/CD115 Antibody (AFS98). Tissue sections were deparaffinized and incubated with the antibody O/N at 4C. IHC-P image submitted by a verified customer review.
Flow Cytometry: M-CSFR/CD115 Antibody (AFS98) - BSA Free [NBP1-43363]

Flow Cytometry: M-CSFR/CD115 Antibody (AFS98) - BSA Free [NBP1-43363]

Flow Cytometry: M-CSF R/CD115 Antibody (AFS98) [NBP1-43363] - Analysis using the Biotin conjugate of NBP1-43363. Staining of thioglycolate-induced peritoneal exudate cells with Anti-Mouse CD115 (c-fms) PE. Appropriate isotype controls were used (open histogram).
Immunohistochemistry-Frozen: M-CSFR/CD115 Antibody (AFS98) - BSA Free [NBP1-43363]

Immunohistochemistry-Frozen: M-CSFR/CD115 Antibody (AFS98) - BSA Free [NBP1-43363]

Immunohistochemistry-Frozen: M-CSFR/CD115 Antibody (AFS98) [NBP1-43363] - Mouse brain section. Antibody at 1:200. IHC-Fr image submitted by a verified customer review.
Flow Cytometry: M-CSFR/CD115 Antibody (AFS98) - BSA Free [NBP1-43363]

Flow Cytometry: M-CSFR/CD115 Antibody (AFS98) - BSA Free [NBP1-43363]

Flow Cytometry: M-CSF R/CD115 Antibody (AFS98) [NBP1-43363] - Staining of thioglycolate-induced peritoneal exudate cells with Anti-Mouse CD115 (c-fms) PE. Appropriate isotype controls were used (open histogram). Cells in the large scatter population were used for analysis.
Flow Cytometry: M-CSFR/CD115 Antibody (AFS98) - BSA Free [NBP1-43363]

Flow Cytometry: M-CSFR/CD115 Antibody (AFS98) - BSA Free [NBP1-43363]

Flow Cytometry: M-CSF R/CD115 Antibody (AFS98) [NBP1-43363] - Staining of thioglycolate-induced peritoneal exudate cells (PECs) with 0.25 ug of Rat IgG2a kappa Isotype Control Purified (NBP1-43321) (open histogram) or 0.25 ug of Anti-Mouse CD115 (c-fms) Purified (filled histogram) followed by Anti-Rat IgG Biotin and Streptavidin PE. Total viable cells were used for analysis.

Applications for M-CSFR/CD115 Antibody (AFS98) - BSA Free

Application
Recommended Usage

Flow Cytometry

< 1 ug/10^6 cells

Immunohistochemistry

1:10 - 1:500

Immunohistochemistry-Frozen

1:10 - 1:500

Western Blot

1:100 - 1:2000
Application Notes
The AFS98 antibody has been tested by blocking of fluorochrome conjugated AFS98 in flow cytometric analysis of peritoneal exudate cells. This can be used at less than or equal to 1 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test. Use in Blocking/Neutralizing reported in scientific literature (PMID: 24056963). This M-CSFR/CD115 Antibody (AFS98) is validated for IHC-P from a verified customer review.

Reviewed Applications

Read 2 reviews rated 4.5 using NBP1-43363 in the following applications:

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS (pH 7.2)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: M-CSF R/CD115

The AFS98 monoclonal antibody reacts with the mouse CD115 molecule, a receptor for macrophage colony stimulating factor (M-CSF) or colony stimulating factor-1 (CSF-1). CD115 is expressed by monocyte, macrophage, osteoclast, and some epithelial cells. It is a 150 kDa c-fms gene product and belongs to immunoglobulin family. CSF-1 signaling through CSF-1R regulates the proliferation and differentiation of cells in the monocytic lineage.

Long Name

Macrophage Colony Stimulating Factor Receptor

Alternate Names

c-fms, CD115, CSF1R, M-CSFR, MCSFR

Entrez Gene IDs

1436 (Human)

Gene Symbol

CSF1R

UniProt

P07333 (Human)

Additional M-CSF R/CD115 Products

Product Documents for M-CSFR/CD115 Antibody (AFS98) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for M-CSFR/CD115 Antibody (AFS98) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for M-CSFR/CD115 Antibody (AFS98) - BSA Free

Customer Reviews for M-CSFR/CD115 Antibody (AFS98) - BSA Free (2)

4.5 out of 5
2 Customer Ratings
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Customer Images

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  • M-CSFR/CD115 Antibody (AFS98)
    Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: Tumor tissue
    Species: Mouse
    Verified Customer | Posted 11/09/2021
    staining in mouse tumor tissue
    Tissue sections were deparaffinized. Incubated O/N in 4 C with the 1ry antibody.
    M-CSFR/CD115 Antibody (AFS98) - BSA Free NBP1-43363
  • M-CSFR/CD115 Antibody (AFS98)
    Name: Abhijit Nirwane
    Application: Immunohistochemistry-Frozen
    Sample Tested: Mouse brain
    Species: Mouse
    Verified Customer | Posted 09/18/2019
    Dilution 1:200
    M-CSFR/CD115 Antibody (AFS98) - BSA Free NBP1-43363

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Protocols

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FAQs

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Associated Pathways

Dendritic Cell Lineage Development Pathways
Dendritic Cell Lineage Development Pathway Thumbnail