MAL Antibody (B5-G3)

Novus Biologicals | Catalog # NBP2-75563

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # B5-G3
View all MAL Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide within mouse MAL aa 97-146 / 153. (SwissProt: P21145 Human; SwissProt: O09198 Mouse; SwissProt: Q64349 Rat)

Reactivity Notes

Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-questions.

Localization

Membrane.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MAL Antibody (B5-G3)

Western Blot: MAL Antibody (B5-G3) [NBP2-75563]

Western Blot: MAL Antibody (B5-G3) [NBP2-75563]

Western Blot: MAL Antibody (B5-G3) [NBP2-75563] - Analysis of MAL on mouse kidney (1) and mouse lymphatic vessels (2) tissue lysate using anti-MAL antibody at 1/1,000 dilution.
Immunohistochemistry-Paraffin: MAL Antibody (B5-G3) [NBP2-75563]

Immunohistochemistry-Paraffin: MAL Antibody (B5-G3) [NBP2-75563]

Immunohistochemistry-Paraffin: MAL Antibody (B5-G3) [NBP2-75563] - Analysis of paraffin-embedded rat kidney tissue using anti-MAL antibody. Counter stained with hematoxylin.
Flow Cytometry: MAL Antibody (B5-G3) [NBP2-75563]

Flow Cytometry: MAL Antibody (B5-G3) [NBP2-75563]

Flow Cytometry: MAL Antibody (B5-G3) [NBP2-75563] - Analysis of Jurkat cells with MAL antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Immunohistochemistry-Paraffin: MAL Antibody (B5-G3) [NBP2-75563]

Immunohistochemistry-Paraffin: MAL Antibody (B5-G3) [NBP2-75563]

Immunohistochemistry-Paraffin: MAL Antibody (B5-G3) [NBP2-75563] - Analysis of paraffin-embedded mouse brain tissue using anti-MAL antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: MAL Antibody (B5-G3) [NBP2-75563]

Immunohistochemistry-Paraffin: MAL Antibody (B5-G3) [NBP2-75563]

Immunohistochemistry-Paraffin: MAL Antibody (B5-G3) [NBP2-75563] - Analysis of paraffin-embedded mouse kidney tissue using anti-MAL antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: MAL Antibody (B5-G3) [NBP2-75563]

Immunohistochemistry-Paraffin: MAL Antibody (B5-G3) [NBP2-75563]

Immunohistochemistry-Paraffin: MAL Antibody (B5-G3) [NBP2-75563] - Analysis of paraffin-embedded rat brain tissue using anti-MAL antibody. Counter stained with hematoxylin.
MAL Antibody (B5-G3) Western Blot: MAL Antibody (B5-G3) [NBP2-75563]

Western Blot: MAL Antibody (B5-G3) [NBP2-75563]

Western blot analysis of Mouse kidney tissue lysate with NBP2-75563 at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 100 seconds Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: NBP2-75563, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat Anti-Rabbit IgG [HRP] Secondary Antibody, 1/5,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 17 kDa Observed band size: 25 kDa

Applications for MAL Antibody (B5-G3)

Application
Recommended Usage

Flow Cytometry

1:50-1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:500-1:1000

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH7.4), 0.2% BSA, 50% Glycerol

Preservative

0.05% Sodium Azide

Concentration

2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MAL

The protein encoded by the MAL gene is a highly hydrophobic integral membrane protein belonging to the MAL family of proteolipids. The protein has been localized to the endoplasmic reticulum of T-cells and is a candidate linker protein in T-cell signal transduction. In addition, this proteolipid is localized in compact myelin of cells in the nervous system and has been implicated in myelin biogenesis and/or function. The protein plays a role in the formation, stabilization and maintenance of glycosphingolipid-enriched membrane microdomains. Alternative splicing produces four transcript variants which vary from each other by the presence or absence of alternatively spliced exons 2 and 3. (provided by RefSeq)

Alternate Names

mal, T-cell differentiation protein, myelin and lymphocyte protein, T-cell differentiation protein MAL, T-lymphocyte maturation-associated protein

Gene Symbol

MAL

Additional MAL Products

Product Documents for MAL Antibody (B5-G3)

Certificate of Analysis

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Product Specific Notices for MAL Antibody (B5-G3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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