MFG-E8 Antibody (EDM45) - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-47813
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Flow Cytometry, Immunofluorescence, Immunocytochemistry/ Immunofluorescence, CyTOF-ready
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # EDM45
Format
Azide and BSA Free
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Product Specifications
Immunogen
Delipidated human milk fat globule membrane preparation
Localization
Cell Surface & Cytoplasmic
Marker
Breast Epithelial Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-44412).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for MFG-E8 Antibody (EDM45) - Azide and BSA Free
![Immunohistochemistry-Paraffin: MFG-E8 Antibody (EDM45) - Azide and BSA Free [NBP2-47813]](https://beta-resources.rndsystems.com/images/products/MFG-E8-Antibody-EDM45-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47813-img0003.jpg "Immunohistochemistry-Paraffin: MFG-E8 Antibody (EDM45) - Azide and BSA Free [NBP2-47813]")
Immunohistochemistry-Paraffin: MFG-E8 Antibody (EDM45) - Azide and BSA Free [NBP2-47813]
Immunohistochemistry-Paraffin: MFG-E8 Antibody (EDM45) - Azide and BSA Free [NBP2-47813] - Formalin-fixed, paraffin-embedded human Pancreas stained with MFG-E8 Antibody (EDM45)![Immunohistochemistry-Paraffin: MFG-E8 Antibody (EDM45) - Azide and BSA Free [NBP2-47813]](https://beta-resources.rndsystems.com/images/products/MFG-E8-Antibody-EDM45-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47813-img0001.jpg "Immunohistochemistry-Paraffin: MFG-E8 Antibody (EDM45) - Azide and BSA Free [NBP2-47813]")
Immunohistochemistry-Paraffin: MFG-E8 Antibody (EDM45) - Azide and BSA Free [NBP2-47813]
Immunohistochemistry-Paraffin: MFG-E8 Antibody (EDM45) - Azide and BSA Free [NBP2-47813] - Formalin-fixed, paraffin-embedded human Lung Carcinoma stained with MFG-E8 Antibody (EDM45)![Immunohistochemistry-Paraffin: MFG-E8 Antibody (EDM45) - Azide and BSA Free [NBP2-47813]](https://beta-resources.rndsystems.com/images/products/MFG-E8-Antibody-EDM45-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47813-img0002.jpg "Immunohistochemistry-Paraffin: MFG-E8 Antibody (EDM45) - Azide and BSA Free [NBP2-47813]")
Immunohistochemistry-Paraffin: MFG-E8 Antibody (EDM45) - Azide and BSA Free [NBP2-47813]
Immunohistochemistry-Paraffin: MFG-E8 Antibody (EDM45) - Azide and BSA Free [NBP2-47813] - Human Breast Carcinoma stained with Milk Fat Globule Monoclonal Antibody (EDM45)![Immunohistochemistry-Paraffin: MFG-E8 Antibody (EDM45) - Azide and BSA Free [NBP2-47813]](https://beta-resources.rndsystems.com/images/products/MFG-E8-Antibody-EDM45-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47813-img0004.jpg "Immunohistochemistry-Paraffin: MFG-E8 Antibody (EDM45) - Azide and BSA Free [NBP2-47813]")
Immunohistochemistry-Paraffin: MFG-E8 Antibody (EDM45) - Azide and BSA Free [NBP2-47813]
Immunohistochemistry-Paraffin: MFG-E8 Antibody (EDM45) - Azide and BSA Free [NBP2-47813] - Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with MFG-E8 Antibody (EDM45)Applications for MFG-E8 Antibody (EDM45) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
0.5 - 1 ug/million cells in 0.1 ml
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunofluorescence
1 - 2 ug/ml
Immunohistochemistry-Paraffin
0.5 - 1.0 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: MFG-E8
Long Name
Milk Fat Globule EGF Factor 8
Alternate Names
BA46, Breast epithelial antigen BA46, Lactahedrin, Medin, MFGE8, SED1
Gene Symbol
MFGE8
Additional MFG-E8 Products
Product Documents for MFG-E8 Antibody (EDM45) - Azide and BSA Free
Product Specific Notices for MFG-E8 Antibody (EDM45) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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