Mouse CD155/PVR Antibody

MAB6909 1 Citation Datasheet / COA / SDS

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MAB6909
MAB6909-SP

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CD155/PVR Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse CD155/PVR in direct ELISAs.

Source

Monoclonal Rat IgG_2A Clone # 690912

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse CD155/PVR
Asp29-Leu348
Accession # NP_081790

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Flow Cytometry

2.5 µg/10⁶ cells

See below

Blockade of Receptor-ligand Interaction

In a functional ELISA binding assay, 0.2-0.8 μg/mL of this antibody will block 50% of the binding of Recombinant Mouse CD155/PVR Fc Chimera (Catalog # 9670-CD) to immobilized RecombinantMouse CD96 (Catalog # 5690-CD) coated at 1 μg/mL (100 μL/well). At 10 μg/mL, this antibody will block >90% of the binding.

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot

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Detection of Mouse CD155/PVR by Western Blot. Western blot shows lysates of TM4 mouse sertoli cell line and mouse small intestine tissue. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse CD155/PVR Monoclonal Antibody (Catalog # MAB6909) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for CD155/PVR at approximately 80 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry

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CD155/PVR in Mouse Thymocytes. CD155/PVR was detected in immersion fixed adult mouse thymocytes using Rat Anti-Mouse CD155/PVR Monoclonal Antibody (Catalog # MAB6909) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Flow Cytometry

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Detection of CD155/PVR in Mouse Thymocytes by Flow Cytometry Mouse thymocytes were stained with Rat Anti-Mouse CD155/PVR Monoclonal Antibody (Catalog # MAB6909, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD155/PVR

CD155, also known as PVR (poliovirus receptor) and Necl-5 (Nectin-like molecule-5), is a 70 kDa type I transmembrane glycoprotein that binds poliovirus as well as Vitronectin, Nectin-3, and DNAM-1. CD155 contains three Ig-like domains in its extracellular region. It is expressed in a variety of normal tissues and is upregulated in tumor cells of epithelial and neuronal origin. Within aa 29-348 of the extracellular region, mouse CD155 shares 45% and 73% aa sequence identity with human and rat CD155, respectively.

Long Name

Poliovirus Receptor

Entrez Gene IDs

5817 (Human); 52118 (Mouse); 25066 (Rat); 102136444 (Cynomolgus Monkey); 712851 (Rhesus Macaque)

Alternate Names

CD155 antigen; CD155; HVED; NECL5; Necl-5; nectin-like 5; Nectin-like protein 5; poliovirus receptor; PVR; PVS; PVSFLJ25946; Tage4

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Mouse CD155/PVR Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1