Mouse CD155/PVR Antibody Summary
Accession # NP_081790
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Mouse CD155/PVR by Western Blot. Western blot shows lysates of TM4 mouse sertoli cell line and mouse small intestine tissue. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse CD155/PVR Monoclonal Antibody (Catalog # MAB6909) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for CD155/PVR at approximately 80 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.
CD155/PVR in Mouse Thymocytes. CD155/PVR was detected in immersion fixed adult mouse thymocytes using Rat Anti-Mouse CD155/PVR Monoclonal Antibody (Catalog # MAB6909) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of CD155/PVR in Mouse Thymocytes by Flow Cytometry Mouse thymocytes were stained with Rat Anti-Mouse CD155/PVR Monoclonal Antibody (Catalog # MAB6909, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
CD155, also known as PVR (poliovirus receptor) and Necl-5 (Nectin-like molecule-5), is a 70 kDa type I transmembrane glycoprotein that binds poliovirus as well as Vitronectin, Nectin-3, and DNAM-1. CD155 contains three Ig-like domains in its extracellular region. It is expressed in a variety of normal tissues and is upregulated in tumor cells of epithelial and neuronal origin. Within aa 29-348 of the extracellular region, mouse CD155 shares 45% and 73% aa sequence identity with human and rat CD155, respectively.
Citation for Mouse CD155/PVR Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Natural killer cells impede the engraftment of cardiomyocytes derived from induced pluripotent stem cells in syngeneic mouse model
Authors: Y Nakamura, S Miyagawa, S Yoshida, S Sasawatari, T Toyofuku, K Toda, Y Sawa
Sci Rep, 2019;9(1):10840.
Sample Types: Whole Cells
Applications: Flow Cytometry
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