Mouse CD155/PVR Antibody

Catalog # Availability Size / Price Qty
MAB6909
MAB6909-SP
Mouse CD155/PVR Antibody in Data
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Product Details
Citations (1)
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Mouse CD155/PVR Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CD155/PVR in direct ELISAs.
Source
Monoclonal Rat IgG2A Clone # 690912
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse CD155/PVR
Asp29-Leu348
Accession # NP_081790
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Flow Cytometry
2.5 µg/106 cells
See below
Blockade of Receptor-ligand Interaction
In a functional ELISA binding assay, 0.2-0.8 μg/mL of this antibody will block 50% of the binding of Recombinant Mouse CD155/PVR Fc Chimera (Catalog # 9670-CD) to immobilized RecombinantMouse CD96 (Catalog # 5690-CD) coated at 1 μg/mL (100 μL/well). At 10 μg/mL, this antibody will block >90% of the binding.
 
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Mouse CD155/PVR antibody by Western Blot. View Larger

Detection of Mouse CD155/PVR by Western Blot. Western blot shows lysates of TM4 mouse sertoli cell line and mouse small intestine tissue. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse CD155/PVR Monoclonal Antibody (Catalog # MAB6909) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for CD155/PVR at approximately 80 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry CD155/PVR antibody in Mouse Thymocytes by Immunocytochemistry (ICC). View Larger

CD155/PVR in Mouse Thymocytes. CD155/PVR was detected in immersion fixed adult mouse thymocytes using Rat Anti-Mouse CD155/PVR Monoclonal Antibody (Catalog # MAB6909) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Flow Cytometry Detection of CD155/PVR antibody in Mouse Thymocytes antibody by Flow Cytometry View Larger

Detection of CD155/PVR in Mouse Thymocytes by Flow Cytometry Mouse thymocytes were stained with Rat Anti-Mouse CD155/PVR Monoclonal Antibody (Catalog # MAB6909, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD155/PVR

CD155, also known as PVR (poliovirus receptor) and Necl-5 (Nectin-like molecule-5), is a 70 kDa type I transmembrane glycoprotein that binds poliovirus as well as Vitronectin, Nectin-3, and DNAM-1. CD155 contains three Ig-like domains in its extracellular region. It is expressed in a variety of normal tissues and is upregulated in tumor cells of epithelial and neuronal origin. Within aa 29-348 of the extracellular region, mouse CD155 shares 45% and 73% aa sequence identity with human and rat CD155, respectively.

Long Name
Poliovirus Receptor
Entrez Gene IDs
5817 (Human); 52118 (Mouse); 25066 (Rat); 102136444 (Cynomolgus Monkey); 712851 (Rhesus Macaque)
Alternate Names
CD155 antigen; CD155; HVED; NECL5; Necl-5; nectin-like 5; Nectin-like protein 5; poliovirus receptor; PVR; PVS; PVSFLJ25946; Tage4

Product Datasheets

Citation for Mouse CD155/PVR Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Natural killer cells impede the engraftment of cardiomyocytes derived from induced pluripotent stem cells in syngeneic mouse model
    Authors: Y Nakamura, S Miyagawa, S Yoshida, S Sasawatari, T Toyofuku, K Toda, Y Sawa
    Sci Rep, 2019;9(1):10840.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

FAQs

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Cell and Tissue Staining Kits

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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