Mouse Plexin C1 Alexa Fluor® 750-conjugated Antibody

Catalog #: AF5375S Datasheet / COA / SDS

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AF5375S-100UG

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Mouse Plexin C1 Alexa Fluor® 750-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Plexin C1 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross‑reactivity with recombinant human Plexin C1 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse Plexin C1
Ala35-Thr950
Accession # Q9QZC2

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 750 (Excitation= 749 nm, Emission= 775 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilution of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

CyTOF-ready

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Plexin C1

Plexin C1 (also VESPR and CD232) is a 200‑210 kDa member of the plexin C subfamily, plexin family of proteins. It is a type I transmembrane glycoprotein that is found on monocytes, dendritic cells, neutrophils and embryonic neuroendocrine neurons of the hyypothalamus. Plexin C1 binds Sema7A, and its viral counterpart, vaccinia semaphorin A39R. Ligation induces actin cytoskeletal rearrangement and cell immobilization. Mature mouse Plexin C1 is 1540 amino acids (aa) in length. It contains a 916 aa extracellular domain that shows one Sema-domain (aa 35‑452) and a 603 aa cytoplasmic tail. There are two potential splice variants that show a five and 67 aa substitution for aa 861‑870 and 1206‑1574, respectively. Over aa 35‑950, mouse Plexin C1 is 95% and 85% aa identical to rat and human Plexin C1, respectively.

Entrez Gene IDs

10154 (Human); 54712 (Mouse); 362873 (Rat)

Alternate Names

CD232 antigen; CD232; plexin (semaphorin receptor); Plexin C1; PLXNC1; PLXN-C1; receptor for viral semaphorin protein; receptor for virally-encoded semaphorin; VESPR; VESPRplexin-C1; Virus-encoded semaphorin protein receptor