Mouse S1P1/EDG-1 Antibody (713412)

Detection of S1P1/EDG-1 by Flow Cytometry

CD69 and S1PR1 expression on CD4 T cells before and following cell transfer. Activated OT-II T cells were analyzed before cell transfer (A) for expression of CD69 and S1PR1 or for CD69 expression ex vivo 24 h after transfer into resting (PBS) or inflamed [lipopolysaccharide (LPS)] ear pinnae (B,C). Before transfer, the OT-II T cells were treated with an S1PR1 antagonist or agonist as in Figure 5B. In panel (A), cells are gated on live CD4+ CD45+ cells; representative of three independent samples. In panels (B,C), data are combined from two to three independent experiments with ≥3 animals per group. In this figure, p < 0.05 is indicated by * and p < 0.001 by **. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/29225602), licensed under a CC-BY license. Not internally tested by R&D Systems.