NCAM-1/CD56 Antibody (RNL-1) - BSA Free

A: Mouse on mouse staining, while not ideal, can still be accomplished. This antibody was raised against the human form of the protein, but most likely shares significant homology with mouse, to where it can also pick up that protein. To eliminate the high background that can occur with same species staining we would recommend directly conjugating the primary antibody to your detection method such as HRP, or a Fluorescent marker, to eliminate the use of a secondary. Without having to use a secondary you won't get non-specific binding of the anti-mouse secondary to your samples. Another way you can achieve this is by blocking endogenous Fc receptors with non-specific antibodies that are mouse. This can be accomplished by incubating your samples with mouse serum ahead of adding your antibodies. This can help block out the Fc receptors and eliminate the background if you plan to use a secondary.