NEK6 Antibody (OTI5D7)

Novus Biologicals | Catalog # NBP1-47865

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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI5D7
View all NEK6 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human NEK6 (NP_055212) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Specificity

This antibody is specific for Homo sapiens NIMA (never in mitosis gene a)-related kinase 6 (NEK6), transcript variant 2.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

35.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NEK6 Antibody (OTI5D7)

Western Blot: NEK6 Antibody (OTI5D7) [NBP1-47865]

Western Blot: NEK6 Antibody (OTI5D7) [NBP1-47865]

Western Blot: NEK6 Antibody (OTI5D7) [NBP1-47865] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NEK6 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NEK6.
Immunocytochemistry/ Immunofluorescence: NEK6 Antibody (OTI5D7) [NBP1-47865]

Immunocytochemistry/ Immunofluorescence: NEK6 Antibody (OTI5D7) [NBP1-47865]

Immunocytochemistry/Immunofluorescence: NEK6 Antibody (OTI5D7) [NBP1-47865] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY NEK6.
Immunohistochemistry-Paraffin: NEK6 Antibody (OTI5D7) [NBP1-47865]

Immunohistochemistry-Paraffin: NEK6 Antibody (OTI5D7) [NBP1-47865]

Immunohistochemistry-Paraffin: NEK6 Antibody (OTI5D7) [NBP1-47865] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-NEK6 mouse monoclonal antibody.
Flow Cytometry: NEK6 Antibody (OTI5D7) [NBP1-47865]

Flow Cytometry: NEK6 Antibody (OTI5D7) [NBP1-47865]

Flow Cytometry: NEK6 Antibody (OTI5D7) [NBP1-47865] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-NEK6 antibody, and then analyzed by flow cytometry.
Immunohistochemistry-Paraffin: NEK6 Antibody (OTI5D7) [NBP1-47865]

Immunohistochemistry-Paraffin: NEK6 Antibody (OTI5D7) [NBP1-47865]

Immunohistochemistry-Paraffin: NEK6 Antibody (OTI5D7) [NBP1-47865] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-NEK6 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: NEK6 Antibody (OTI5D7) [NBP1-47865]

Immunohistochemistry-Paraffin: NEK6 Antibody (OTI5D7) [NBP1-47865]

Immunohistochemistry-Paraffin: NEK6 Antibody (OTI5D7) [NBP1-47865] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-NEK6 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: NEK6 Antibody (OTI5D7) [NBP1-47865]

Immunohistochemistry-Paraffin: NEK6 Antibody (OTI5D7) [NBP1-47865]

Immunohistochemistry-Paraffin: NEK6 Antibody (OTI5D7) [NBP1-47865] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-NEK6 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: NEK6 Antibody (OTI5D7) [NBP1-47865]

Immunohistochemistry-Paraffin: NEK6 Antibody (OTI5D7) [NBP1-47865]

Immunohistochemistry-Paraffin: NEK6 Antibody (OTI5D7) [NBP1-47865] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-NEK6 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: NEK6 Antibody (OTI5D7) [NBP1-47865]

Immunohistochemistry-Paraffin: NEK6 Antibody (OTI5D7) [NBP1-47865]

Immunohistochemistry-Paraffin: NEK6 Antibody (OTI5D7) [NBP1-47865] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-NEK6 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: NEK6 Antibody (OTI5D7) [NBP1-47865]

Immunohistochemistry-Paraffin: NEK6 Antibody (OTI5D7) [NBP1-47865]

Immunohistochemistry-Paraffin: NEK6 Antibody (OTI5D7) [NBP1-47865] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-NEK6 mouse monoclonal antibody.

Applications for NEK6 Antibody (OTI5D7)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Western Blot

1:2000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.75 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NEK6

The Aspergillus nidulans 'never in mitosis A' (NIMA) gene encodes a serine/threonine kinase that controls initiation of mitosis. NIMA-related kinases (NEKs) are a group of protein kinases that are homologous to NIMA. Evidence suggests that NEKs perform functions similar to those of NIMA.

Alternate Names

EC 2.7.11.1, Never in mitosis A-related kinase 6, NIMA (never in mitosis gene a)-related kinase 6, NimA-related protein kinase 6, Protein kinase SID6-1512, putative serine-threonine protein kinase, serine/threonine-protein kinase Nek6, SID6-1512

Entrez Gene IDs

10783 (Human)

Gene Symbol

NEK6

UniProt

Q9HC98 (Human)

Additional NEK6 Products

Product Documents for NEK6 Antibody (OTI5D7)

Certificate of Analysis

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Product Specific Notices for NEK6 Antibody (OTI5D7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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